Evidence map›Paper›PMID 40043123›Full record

ArticleScience advances2025

Scalable acoustic virtual stirrer for enhanced interfacial enzymatic nucleic acid reactions.

Dayang Li, Kunjie Li, Jianquan Li, Dongfang Li, Heng Chen, Sen-Sen Li, Chaoyong Yang, Huimin Zhang, Lu-Jian Chen, Xuejia Hu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Dayang LiSchool of Electronic Science and Engineering, Fujian Key Laboratory of Ultrafast Laser Technology and Applications, Xiamen University, Xiamen 361012, P. R. China.ORCID 0009-0009-7383-2207
Kunjie LiKey Laboratory of Spectrochemical Analysis and Instrumentation, Ministry of Education, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen 361102, China.ORCID 0000-0002-5843-3912
Jianquan LiSchool of Electronic Science and Engineering, Fujian Key Laboratory of Ultrafast Laser Technology and Applications, Xiamen University, Xiamen 361012, P. R. China.
Dongfang LiSchool of Electronic Science and Engineering, Fujian Key Laboratory of Ultrafast Laser Technology and Applications, Xiamen University, Xiamen 361012, P. R. China.
Heng ChenKey Laboratory of Spectrochemical Analysis and Instrumentation, Ministry of Education, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen 361102, China.ORCID 0009-0005-6937-3774
Sen-Sen LiSchool of Electronic Science and Engineering, Fujian Key Laboratory of Ultrafast Laser Technology and Applications, Xiamen University, Xiamen 361012, P. R. China.
Chaoyong YangKey Laboratory of Spectrochemical Analysis and Instrumentation, Ministry of Education, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen 361102, China.ORCID 0000-0002-2374-5342
Huimin ZhangKey Laboratory of Spectrochemical Analysis and Instrumentation, Ministry of Education, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen 361102, China.ORCID 0000-0002-7343-5641
Lu-Jian ChenSchool of Electronic Science and Engineering, Fujian Key Laboratory of Ultrafast Laser Technology and Applications, Xiamen University, Xiamen 361012, P. R. China.ORCID 0000-0002-4156-019X
Xuejia HuSchool of Electronic Science and Engineering, Fujian Key Laboratory of Ultrafast Laser Technology and Applications, Xiamen University, Xiamen 361012, P. R. China.ORCID 0000-0001-8891-2140

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enzymatic nucleic acid reaction is a fundamental tool in molecular biology. However, high-complexity enzymatic DNA reactions and assays are still challenging due to the difficulties in integrating and scaling up microscale reaction units and mixing tools. Here, we present scalable acoustofluidic platform featuring acoustic virtual stirrer (AVS) arrays, serving as stirrers to increase the efficiency of interfacial enzymatic nucleic acid reactions. Analogous to magnetic stirrers, AVS arrays perturb the fluid through oscillating pressure nodes, controllable in terms of speeds and amplitudes via modulation. By optimizing the kinetics of surface-tethered DNA and enzymes via AVS, we achieve a 7.74% improvement in the stepwise yield of enzymatic DNA synthesis. In addition, the AVS enhanced DNA logic gate architecture can complete responses within 2 minutes, achieving average speed enhancement of 8.58 times compared to the non-AVS configuration. With its tunability, ease of integration, and efficiency, this technology holds promises for applications in biology and chemistry.

Indexed as

AcousticsDNANucleic AcidsKineticsDNANucleic Acids

Identifiers

PMID40043123
PMCPMC11881892

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.