Evidence map›Paper›PMID 40042671›Full record

ArticleEuropean journal of nutrition2025

Oral intake of Hibiscus sabdariffa L. increased c-Myc and caspase-3 gene expression and altered microbial population in colon of BALB/c mice induced to preneoplastic lesions.

Andressa Ladeira Bernardes, Mariana de Fátima Albuquerque Pereira, Iasmim Xisto Campos, Larissa Ávila, Bruna Cristina Dos Santos Cruz, Marcella Duarte Villas Mishima, Manoela Maciel Dos Santos Dias, Tiago Antônio de Oliveira Mendes, Maria do Carmo Gouveia Peluzio

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Article in European journal of nutrition, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Andressa Ladeira BernardesDepartment of Nutrition and Health, Universidade Federal de Viçosa, Campus Universitário da, Viçosa, Minas Gerais, CEP:36570-900, Brazil. andressa.bernardes@ufv.br.ORCID http://orcid.org/0000-0003-0972-5780
Mariana de Fátima Albuquerque PereiraDepartment of Nutrition and Health, Universidade Federal de Viçosa, Campus Universitário da, Viçosa, Minas Gerais, CEP:36570-900, Brazil.
Iasmim Xisto CamposDepartment of Nutrition and Health, Universidade Federal de Viçosa, Campus Universitário da, Viçosa, Minas Gerais, CEP:36570-900, Brazil.
Larissa ÁvilaDepartment of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil.
Bruna Cristina Dos Santos CruzDepartment of Nutrition and Health, Universidade Federal de Viçosa, Campus Universitário da, Viçosa, Minas Gerais, CEP:36570-900, Brazil.
Marcella Duarte Villas MishimaDepartment of Nutrition and Health, Universidade Federal de Viçosa, Campus Universitário da, Viçosa, Minas Gerais, CEP:36570-900, Brazil.
Manoela Maciel Dos Santos DiasDepartment of Nutrition and Health, Universidade Federal de Viçosa, Campus Universitário da, Viçosa, Minas Gerais, CEP:36570-900, Brazil.
Tiago Antônio de Oliveira MendesDepartment of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil.
Maria do Carmo Gouveia PeluzioDepartment of Nutrition and Health, Universidade Federal de Viçosa, Campus Universitário da, Viçosa, Minas Gerais, CEP:36570-900, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundColorectal carcinogenesis induces changes in the colon, such as the appearance of aberrant crypt foci (ACF). This process is influenced by genetic and environmental factors, such as diet. Hibiscus sabdariffa L. is a considerable source of phenolic compounds, such as anthocyanins, and dietary fibers that may exert anti-inflammatory, antioxidant and prebiotic properties, attenuating the appearance of ACFs.

objectivesTo investigate whether supplementation with 5% or 10% of dehydrated calyces of Hibiscus sabadariffa (DHSC) influences the composition of the intestinal microbiota and the expression of genes related to colorectal carcinogenesis in BALB/c mice.

methodsThe in vivo experiment lasted 12 weeks and the animals were divided into 3 experimental groups: the control group and the supplemented groups (5% or 10% DCHS) and induced pre-neoplastic lesions with the drug Dimethylhydrazine. Serum aspartate aminotransferase and alanine aminotransferase markers, liver cytokine profile, gut microbiota composition and tumor protein 53, cellular myelocytomatosis oncogene, caspase-3 and Proliferating Cell Nuclear Antigen gene expression were determined.

resultsSupplementation with 5% or 10% of DCHS altered the composition of the intestinal microbiota, increasing the abundance of the families Lachnospiraceae, Ruminococcaceae, Clostridiaceae and of the genus Clostridum, important producers of butyrate. Furthermore, 5% and 10% DCHS supplementation increased caspase-3 and c-Myc expression, respectively, which may suggest apoptotic events.

conclusionsTherefore, the effects of DHSC, rich in anthocyanins and dietary fiber, on the composition of the intestinal microbiota and on the expression of genes associated with cell apoptosis may contribute to reducing the risk of developing preneoplastic lesions.

Indexed as

Caspase 3ColonGastrointestinal MicrobiomeHibiscusPrecancerous ConditionsProto-Oncogene Proteins c-mycAnimalsColorectal NeoplasmsDietary SupplementsMaleMiceMice, Inbred BALB CPlant ExtractsCasp3 protein, mouseCaspase 3Myc protein, mousePlant ExtractsProto-Oncogene Proteins c-mycAnthocyaninsCarcinogenesisColonHibiscusPhenolic compoundsPrebiotics

Identifiers

PMID40042671

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.