Evidence map›Paper›PMID 40040808›Full record

ArticleiScience2025

A microfluidic platform for anterior-posterior human endoderm patterning via countervailing morphogen gradients

Leeya Engel, Kevin J Liu, Kiara W Cui, Eva L de la Serna, Vipul T Vachharajani, Carolyn E Dundes, Sherry Li Zheng, Manali Begur, Kyle M Loh, Lay Teng Ang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Leeya EngelDepartment of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
Kevin J LiuInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA.
Kiara W CuiDepartment of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
Eva L de la SernaDepartment of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
Vipul T VachharajaniDepartment of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.
Carolyn E DundesInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA.
Sherry Li ZhengInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA.
Manali BegurInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA.
Kyle M LohInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA.
Lay Teng AngInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA.
Alexander R DunnDepartment of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.

Funding

Molecular mechanisms underlying force transduction at cellular adhesion complexesR35GM130332 · NIGMS · STANFORD UNIVERSITY · PI Alexander R Dunn · 2019 to 2026
$4.9M
NIGMS NIH HHS R35 GM130332
6 · The paper itself

Abstract

Understanding how morphogen gradients spatially pattern tissues is a fundamental question in developmental biology but can be difficult to directly address using conventional approaches. Here, we expose hPSC-derived endoderm cells to countervailing gradients of anteriorizing and posteriorizing signals using a widely available microfluidic device. This approach yielded spatially patterned cultures comprising anterior foregut (precursor to the thyroid, esophagus, and lungs) and mid/hindgut (precursor to the intestines) cells, whose identities were confirmed using single-cell RNA sequencing (scRNA-seq). By exposing stem cells to externally applied signaling gradients, this widely accessible microfluidic platform should accelerate the production of spatially patterned tissues, complementing internally self-organizing organoids. Applying artificial morphogen gradients

Indexed as

Cell biologyDevelopmental biologyFluidicsMethodology in biological sciencesStem cells research

Identifiers

PMID40040808
PMCPMC11879597

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.