ArticleBMC biotechnology2025
Extract optimization of Ulva lactuca L. and biological activities of optimized extracts.
Article in BMC biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed.
- The Inclusion of Supercritical Fluid Extract ofAntioxidants (Basel, Switzerland) · 2026Article
- Evaluation of the Antioxidative and Hepatoprotective Activity ofFoods (Basel, Switzerland) · 2026Article
- Comparative Study of Sulfated Polysaccharides From Ulva Lactuca Grown in Tunisia and Morocco: in Vitro Antioxidant Activity and in Vivo Anti-Inflammatory and Anti-Ulcer Effects.Chemistry & biodiversity · 2026Article
- Ulvan Polysaccharide ofInternational journal of food science · 2026Article
- Advances in the GenusPlants (Basel, Switzerland) · 2025Review
- Artificial intelligence-assisted optimization of Eichhornia crassipes extracts and evaluation of their biological activities.Scientific reports · 2025Article
- Optimizing Ultrasonic-Assisted Extraction Process ofMolecules (Basel, Switzerland) · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlgae are microscopic or macroscopic organisms that can photosynthesize and are found in both freshwater and saltwater ecosystems and are considered one of the cornerstones of life. In our study, the biological activities of extracts produced under the extract conditions that provided the highest biological activity of Ulva lactuca L. were determined.
methodsTwo different methods, Response Surface Method (RSM) and Artificial Neural Network-Genetic Algorithm (ANN-GA) integration were used for optimization. In this context, the optimum extract conditions were determined as 54.940 ˚C temperature, 6.513 h, 42.109 ethanol/water ratio according to the RSM method and 56.286 ˚C temperature, 7.2793 h, 36.8625 ethanol/water ratio according to ANN-GA. The antioxidant activities of the optimized extracts were evaluated by Rel Assay kits, DPPH and FRAP methods. Anticholinesterase activities were determined by testing against acetylcholinesterase and butyrylcholinesterase enzymes. In addition, antiproliferative effects were examined in A549 lung cancer cell line and phenolic compound contents were analyzed by LC-MS/MS.
resultsAs a result of the analyzes, it was seen that the extract obtained from the conditions recommended by ANN-GA exhibited higher activities. Optimized extracts of U. lactuca were found to have potential in terms of antioxidant activities. The highest total antioxidant value was determined as 6.272 ± 0.024 mmol/L. In addition, it was determined that extracts of U. lactuca produced under optimum conditions showed strong cytotoxic effects against A549 lung cancer cell line. In addition, gallic acid, 4-hydroxybenzoic acid, caffeic acid, vanillic acid, syringic acid, quercetin and kaempferol were found in the extracts of the algae produced under optimum conditions. The highest detected compound was caffeic acid. In addition to all these properties, it was seen that U. lactuca has anticholinesterase potential in our study.
conclusionIn conclusion, optimized extracts of U. lactuca attract attention with their antioxidant and cytotoxic activities as well as anticholinesterase potential and can be considered as a potential source for cancer treatment and management of neurological diseases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.