Evidence map›Paper›PMID 40038600›Full record

ArticleBMC immunology2025

In vitro differentiation of common lymphoid progenitor cells into B cell using stromal cell free culture system.

Pan Yang, Xiaoling Chen, Hao Wen, Meiling Yu, Haili Yu, Li Wang, Liang Gong, Lintao Zhao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. CD127International journal of molecular sciences · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Pan Yang *Department of Respiratory And Critical Care Medicine, First Affiliated Hospital of Army Medical University, No. 30 Gaotan Yanzheng Street, Chongqing, 400038, China.
Xiaoling Chen *Institute of Immunology PLA, Army Medical University, No. 30 Gaotan Yanzheng Street, Chongqing, 400038, China.
Hao WenInstitute of Immunology PLA, Army Medical University, No. 30 Gaotan Yanzheng Street, Chongqing, 400038, China.
Meiling YuInstitute of Immunology PLA, Army Medical University, No. 30 Gaotan Yanzheng Street, Chongqing, 400038, China.
Haili YuBiomedical Analysis Center PLA, Army Medical University, Chongqing, 400038, China.
Li WangInstitute of Immunology PLA, Army Medical University, No. 30 Gaotan Yanzheng Street, Chongqing, 400038, China. liwang118@tmmu.edu.cn.
Liang GongDepartment of Respiratory And Critical Care Medicine, First Affiliated Hospital of Army Medical University, No. 30 Gaotan Yanzheng Street, Chongqing, 400038, China. gongliang@tmmu.edu.cn.
Lintao ZhaoDepartment of Respiratory And Critical Care Medicine, First Affiliated Hospital of Army Medical University, No. 30 Gaotan Yanzheng Street, Chongqing, 400038, China. zhao72@tmmu.edu.cn.

Funding

National Nature Science Foundation of China No. 82073169
6 · The paper itself

Abstract

The OP9 culture system is an important in vitro model for B cell development. However, the complex nature of operations and the intrinsic variability of stromal cell functionality, which can be influenced by factors such as radiation exposure or contamination, pose considerable challenges to their wider application. Currently, there exists a paucity of studies documenting in vitro B cell differentiation culture systems that exclude stromal cells, and the experimental methodologies available for reference remain limited. This report elucidates a robust stromal cell-free culture system. Specifically, bovine serum albumin (BSA) or fetal bovine serum (FBS), in conjunction with interleukin-7 (IL-7), Flt3 ligand (Flt3L), and stem cell factor (SCF), were incorporated into X-VIVO15 medium. This system proficiently facilitates the directed differentiation of common lymphoid progenitor cells (CLP), defined as lineage-CD127 + CD117

Indexed as

B-LymphocytesCell Culture TechniquesCell DifferentiationLymphoid Progenitor CellsAnimalsCells, CulturedInterleukin-7Membrane ProteinsMiceStem Cell FactorStromal Cellsflt3 ligand proteinInterleukin-7Membrane ProteinsStem Cell FactorB cellCommon lymphoid progenitor cellsstromal cell free culture systemVitro differentiation

Identifiers

PMID40038600
PMCPMC11877881

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.