ArticleBMC microbiology2025
Comparative in silico and in vivo study of the antioxidant activity of lactoferrin, Geobacillus stearothermophilus, and Lactobacillus delbrueckii subsp. lactis against Rotavirus infection in male mice.
Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rotavirus is a major cause of pediatric gastroenteritis, for which effective treatments are limited. This study investigates the antioxidant and antiviral potential of lactoferrin, Geobacillus stearothermophilus, and Lactobacillus delbrueckii subsp. lactis against Rotavirus infection. In this study, Geobacillus stearothermophilus and Lactobacillus delbrueckii subsp. lactis were isolated from Hammam Pharon soil and milk cheese, respectively, and identified using molecular techniques with accession numbers PP758390 and PP758383. The antioxidant effect against DPPH showed that lactoferrin exhibited the strongest scavenging ability, followed by Geobacillus stearothermophilus and Lactobacillus delbrueckii subsp. lactis. In vivo experiments involved administering lactoferrin, Geobacillus stearothermophilus, and Lactobacillus delbrueckii subsp. lactis in the drinking water of young mice for three days, followed by Rotavirus infection on the fourth day and sacrifice on the fifth day. The results demonstrated that lactoferrin significantly reduced the pathogenic effects of Rotavirus, as indicated by the normalization of inflammatory cytokines (TNF-α and IL-6) in the serum (p ≤ 0.001). Histological examination of small intestinal sections from Rotavirus-infected mice revealed extensive destruction of villus structures, while mice treated with lactoferrin showed no pathological changes compared to the control group. Geobacillus stearothermophilus-treated mice exhibited less pathological alteration and Lactobacillus delbrueckii subsp. lactis-treated mice showed mild pathological changes. Additionally, molecular docking studies indicated that bacteriocin (a bacterial protein) exhibited the highest binding affinity for the Rotavirus outer membrane protein (VP6) at -261.92 kcal/mol, outperforming lactoferrin (-229.32 kcal/mol). Additionally, bacteriocin's active compounds, turimicin (-7.9 kcal/mol) and lactin (-6.5 kcal/mol), also showed strong binding to VP6, suggesting their potential as therapeutic agents against Rotavirus. In conclusion, this study highlights the significant antiviral potential of lactoferrin against Rotavirus, demonstrating its ability to mitigate pathological changes and normalize inflammatory responses in infected mice. The findings also suggest that bacteriocins, particularly those with high binding affinities to Rotavirus proteins, could serve as promising candidates for therapeutic interventions against Rotavirus infections.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.