Evidence map›Paper›PMID 40038411›Full record

ArticleScientific reports2025

Neuroglobin regulates autophagy through mTORC1/RAPTOR/ULK-1 pathway in human neuroblastoma cells.

Valeria Manganelli, Michele Costanzo, Daniela Caissutti, Illari Salvatori, Niccolò Candelise, Emiliano Montalesi, Giovanna De Simone, Alberto Ferri, Tina Garofalo, Maurizio Sorice and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Valeria Manganelli *Department of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.ORCID http://orcid.org/0000-0001-8517-4533
Michele Costanzo *Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples, 80131, Italy.ORCID http://orcid.org/0000-0002-6386-2009
Daniela CaissuttiDepartment of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.ORCID http://orcid.org/0000-0002-5054-8061
Illari SalvatoriDepartment of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.ORCID http://orcid.org/0000-0002-8397-8500
Niccolò CandeliseNational Center for Drug Research and Evaluation, Italian National Institute of Health (ISS), Rome, 00161, Italy.ORCID http://orcid.org/0000-0001-5086-3996
Emiliano MontalesiDepartment of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.ORCID http://orcid.org/0000-0001-8363-6052
Giovanna De SimoneDepartment of Sciences, University of Rome "Roma Tre", Rome, 00146, Italy.ORCID http://orcid.org/0000-0002-9358-9891
Alberto FerriSanta Lucia Foundation IRCCS, Rome, 00179, Italy.ORCID http://orcid.org/0000-0002-4097-0605
Tina GarofaloDepartment of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.ORCID http://orcid.org/0000-0003-0645-2510
Maurizio SoriceDepartment of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.ORCID http://orcid.org/0000-0003-3534-1502
Margherita RuoppoloDepartment of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples, 80131, Italy.ORCID http://orcid.org/0000-0002-7364-0602
Agostina LongoDepartment of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy.ORCID http://orcid.org/0000-0002-1384-4232
Roberta MisasiDepartment of Experimental Medicine, Sapienza University of Rome, Rome, 00161, Italy. roberta.misasi@uniroma1.it.ORCID http://orcid.org/0000-0002-1181-6190

Funding

European Union-Next Generation EU, Mission 4 Component 2 Investment 1.5 Rome Technopole-code ECS 00000024 (CUP: B83C22002820006)Istituto Superiore di Sanità Italy Starting Grant for young researchers 2021, File CB83; CUP I83C22002190005Italian Ministry of Education, University and Research (MIUR) PRIN-PNRR (P2022LZP9T)Italian Ministry of Education, University and Research (MIUR) PRIN 2022 20223RXEECItalian Ministry of Education, University and Research (MIUR) PRIN 2022 2022APEBMYItalian Ministry of Education, University and Research (MIUR) PRIN 2022 2022XL4TE9_003
6 · The paper itself

Abstract

Neuroglobin (NGB) is a hexacoordinated hemeprotein mainly expressed in neurons. Following its upregulation and mitochondrial localization, NGB plays a pro-survival role against neuronal stress. Previously, we built a stable NGB-FLAG-overexpressing neuroblastoma cell line and showed that NGB promotes autophagy and localizes in autophagolysosomes. Here we studied the interactome of NGB-FLAG cells to identify novel autophagy-related NGB-binding partners and investigate how its upregulation could induce autophagy. LC3-II and p62 levels as well as mTORC1 activity were analyzed to evaluate autophagy in NGB-FLAG cells. NGB interactors were identified by affinity purification-mass spectrometry and protein-protein interaction network analysis and validated by immunoprecipitation. The increase of LC3-II and decrease of p62 in NGB-FLAG compared to control confirmed that NGB overexpression promotes autophagy. Interactome analysis identified the Regulatory associated protein of mTOR (RPTOR) as one of 134 putative NGB interactors, further validated by immunoprecipitation. NGB overexpression also determined a consistent increment of RPTOR phosphorylation at Ser792 which is required for mTORC1 inhibition, then confirmed by lower levels of phospho-mTOR and phospho-ULK1 in NGB-FLAG compared to control. Collectively, our data suggests that NGB is a positive regulator of autophagy. Through association with RPTOR, NGB may promote its activation and inhibit mTORC1 repressive activity on autophagy initiation.

Indexed as

AutophagyAutophagy-Related Protein-1 HomologMechanistic Target of Rapamycin Complex 1NeuroblastomaNeuroglobinRegulatory-Associated Protein of mTORSignal TransductionCell Line, TumorHumansIntracellular Signaling Peptides and ProteinsAutophagy-Related Protein-1 HomologIntracellular Signaling Peptides and ProteinsMechanistic Target of Rapamycin Complex 1NeuroglobinRegulatory-Associated Protein of mTORRPTOR protein, humanULK1 protein, humanAutophagyNeuroblastoma cellsNeuroglobinRaptor

Identifiers

PMID40038411
PMCPMC11880548

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.