Evidence map›Paper›PMID 40038265›Full record

ArticleCell death discovery2025

CeDaD-a novel assay for simultaneous tracking of cell death and division in a single population.

Lukas Nöltner, Kurt Engeland, Robin Kohler

Abstract read
In one paragraph

Article in Cell death discovery, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Lukas Nöltner *Molecular Oncology, Faculty of Medicine, University of Leipzig, Leipzig, Germany.ORCID http://orcid.org/0009-0004-1578-5944
Kurt EngelandMolecular Oncology, Faculty of Medicine, University of Leipzig, Leipzig, Germany.ORCID http://orcid.org/0000-0003-3525-0440
Robin Kohler *Molecular Oncology, Faculty of Medicine, University of Leipzig, Leipzig, Germany. Robin.Kohler@medizin.uni-leipzig.de.ORCID http://orcid.org/0009-0000-7728-6308

Funding

Deutsche Forschungsgemeinschaft (German Research Foundation) 424870812
6 · The paper itself

Abstract

The cell division cycle and the various forms of programmed cell death are interconnected. A prominent example is the tumor suppressor p53, which not only induces apoptosis but also plays an important role in the arrest of the cell cycle. Consequently, simultaneous analysis of cell division and cell death is frequently of significant interest in cell biology research. Traditionally, these processes require distinct assays, making concurrent analysis challenging. To address this, we present a novel combined assay, called CeDaD assay-Cell Death and Division assay-which allows for the simultaneous quantification of cell division and cell death within a single-cell population. This assay utilizes a straightforward flow cytometric approach, combining a staining based on carboxyfluorescein succinimidyl ester (CFSE) to monitor cell division with an annexin V-derived staining to assess the extent of cell death.

Identifiers

PMID40038265
PMCPMC11880512

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.