ArticleCardiovascular research2025
Evidence for intermittent coupling of intramyocardial small, engineered heart tissues acutely implanted into rabbit myocardium.
Article in Cardiovascular research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- Accelerated Intrinsic Beating Rate in Heterogeneously Coupled Human Pluripotent Stem Cell-Derived Cardiomyocytes Can Underlie Focal Ventricular Tachycardia in Regenerative Therapy.Journal of precision medicine (Amsterdam, Netherlands) · 2026Article
- Prioritizing Discovery and Advancements in Arrhythmia Therapies: NIH/NHLBI Workshop.JACC. Clinical electrophysiology · 2026Review
- Advanced in vitro cardiac models for drug evaluation: integration of organoids, engineered tissues, and microphysiological systems.Microsystems & nanoengineering · 2026Review
- Phase resetting in human stem cell derived cardiomyocytes explains complex cardiac arrhythmias.PLoS computational biology · 2026Article
Corrections and comments
- Commented on by
Authors and funding
7 authors.
Funding
Abstract
aimsElectrical integration of human-induced pluripotent stem-cell-derived cardiomyocyte (hiPSC-CM)-based tissue with the host myocardium is a requirement of successful regeneration therapy. This study was designed to identify electrical coupling in the acute phase (1-2 h) post-grafting using an ex vivo model. METHODS AND
resultsSmall, engineered heart tissues (mini-EHTs), consisting of ∼50 000 hiPSC-CMs on a hydrogel (spontaneous rate 0.34 ± 0.05 Hz), were loaded with Cal520-AM. EHTs were implanted sub-epicardially into a Langendorff-perfused rabbit heart after blebbistatin treatment. For up to 100 min, a continuous pseudo-electrocardiogram was recorded during sinus rhythm (rate 2.0-3.5 Hz). At 25 min intervals, EHT calcium transients (CaTs) were recorded for 10-20 s (no contraction group). To study the influence of mechanical activity, blebbistatin was washed off after implantation (contraction recovery group). Periodic entrainment of EHTs with the myocardium was detected less often (P = 0.011) in the no contraction group (1/9 hearts) than in the contraction recovery group (5/6 hearts). The average coupling delay (QRS-CaT) and the difference in consecutive delays (Δdelay) were 89 ± 50 and 10 ± 3 ms, respectively (n = 12 traces; N = 6 hearts). Coupling ratios (QRS:CaT) varied from 2:1 to 4:1. These coupling parameters were not significantly different in the two experimental groups. Modelling of hiPSC-CM tissue separated by a 25 μm saline gap from the myocardium demonstrated field-effect coupling with similarly variable activation delays. Importantly, coupling failed with a gap of 100 μm.
conclusionEHT entrainment is possible immediately after grafting and has features compatible with field-effect coupling. Sensitivity to the gap dimensions may explain why entrainment is more common in actively contracting myocardium.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.