Evidence map›Paper›PMID 40036400›Full record

ArticleThe Journal of infectious diseases2025

An Omics-Guided Investigation of a Hospital Outbreak Caused by blaNDM-1-Producing Pseudocitrobacter faecalis.

Roberto B M Marano, Yonatan Oster, Shmuel Benenson, Yair Motro, Oshrat Ayalon, Chaggai Rosenbluh, Aline Cuénod, Ayelet Michael-Gayego, Violeta Temper, Jacob Strahilevitz and 1 more

Abstract read
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In one paragraph

Article in The Journal of infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Roberto B M MaranoDepartment of Health Policy and Management, School of Public Health, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel.ORCID 0000-0001-7805-6360
Yonatan OsterClinical Microbiology Laboratory, Department of Clinical Microbiology and Infectious Diseases, Hadassah Hebrew University Medical Center, Jerusalem, Israel.ORCID 0000-0002-1050-1648
Shmuel BenensonFaculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.ORCID 0009-0006-5813-9701
Yair MotroDepartment of Health Policy and Management, School of Public Health, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel.
Oshrat AyalonClinical Microbiology Laboratory, Department of Clinical Microbiology and Infectious Diseases, Hadassah Hebrew University Medical Center, Jerusalem, Israel.
Chaggai RosenbluhDepartment of Genetics, Hadassah Hebrew University Medical Center, Jerusalem, Israel.
Aline CuénodApplied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland.
Ayelet Michael-GayegoClinical Microbiology Laboratory, Department of Clinical Microbiology and Infectious Diseases, Hadassah Hebrew University Medical Center, Jerusalem, Israel.
Violeta TemperClinical Microbiology Laboratory, Department of Clinical Microbiology and Infectious Diseases, Hadassah Hebrew University Medical Center, Jerusalem, Israel.
Jacob StrahilevitzClinical Microbiology Laboratory, Department of Clinical Microbiology and Infectious Diseases, Hadassah Hebrew University Medical Center, Jerusalem, Israel.ORCID 0000-0002-9192-7164
Jacob Moran-GiladDepartment of Health Policy and Management, School of Public Health, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel.ORCID 0000-0001-9134-050X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Carbapenemase-producing Enterobacterales (CPE) pose a major healthcare challenge. We report the first hospital outbreak of Pseudocitrobacter faecalis carrying blaNDM-1 using an omics-based approach. Short- and long-read sequencing enabled genomic epidemiological investigation to track its spread, characterize its resistome, and analyze the genomic context of blaNDM-1. Additionally, we developed and implemented a matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS)-based method for rapid outbreak isolate typing using protein biomarkers. Our investigation identified 2 independent blaNDM-1-producing clonal clusters of multidrug- and carbapenem-resistant P faecalis circulating for over 3 years, carrying blaNDM-1 either chromosomally or on a plasmid. MALDI-TOF MS spectra analysis revealed candidate protein markers corresponding to genomic clusters, with 1 predicted biomarker applicable for rapid typing. Pseudocitrobacter faecalis is an emerging CPE taxon requiring hospital surveillance. Early whole genome sequencing unexpectedly revealed 2 intertwined clones with independent carbapenemase acquisition routes. Cluster-specific markers enabled rapid typing, serving as proof of concept for validating proteomics in future surveillance.

Indexed as

beta-LactamasesCross InfectionDisease OutbreaksAnti-Bacterial AgentsBacterial ProteinsDrug Resistance, Multiple, BacterialGenome, BacterialGenomicsHospitalsHumansMaleMicrobial Sensitivity TestsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationWhole Genome SequencingAnti-Bacterial AgentsBacterial Proteinsbeta-lactamase NDM-1beta-Lactamasesbla NDM-1carbapenemasesgenomicsoutbreakproteomicsPseudocitrobacter faecalis

Identifiers

PMID40036400
PMCPMC12308669

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.