Evidence map›Paper›PMID 40036265›Full record

ArticleJournal of proteome research2025

Limelight: An Open, Web-Based Tool for Visualizing, Sharing, and Analyzing Mass Spectrometry Data from DDA Pipelines.

Michael Riffle, Alex Zelter, Daniel Jaschob, Michael R Hoopmann, Danielle A Faivre, Robert L Moritz, Trisha N Davis, Michael J MacCoss, Nina Isoherranen

Abstract read
In one paragraph

Article in Journal of proteome research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Michael R HoopmannInstitute for Systems Biology, Seattle, Washington 98109, United States.ORCID 0000-0001-7029-7792
Danielle A FaivreORCID 0000-0003-0535-111X
Robert L MoritzInstitute for Systems Biology, Seattle, Washington 98109, United States.ORCID 0000-0002-3216-9447
Trisha N Davis
Michael J MacCossORCID 0000-0003-1853-0256

Funding

TrainingP41GM103533 · NIGMS · UNIVERSITY OF WASHINGTON · PI DAVIS, TRISHA N. · 2012 to 2021
$20.7M
Shortening the development cycle time of Trans Proteomic Pipeline tools with high performance computingR01GM087221 · NIGMS · INSTITUTE FOR SYSTEMS BIOLOGY · PI DEUTSCH, ERIC, MORITZ, ROBERT L · 2010 to 2021
$6.0M
Molecular Analysis of Chromosome SegregationR35GM130293 · NIGMS · UNIVERSITY OF WASHINGTON · PI DAVIS, TRISHA N. · 2019 to 2023
$3.5M
Identification and quantification of drug-protein adducts by mass spectrometryR01GM147947 · NIGMS · UNIVERSITY OF WASHINGTON · PI ISOHERRANEN, NINA · 2022 to 2025
$1.8M
Acquisition of Fusion Lumos Orbitrap mass spectrometerS10OD026936 · OD · INSTITUTE FOR SYSTEMS BIOLOGY · PI MORITZ, ROBERT L · 2019 to 2019
$600k
NIGMS NIH HHS P41 GM103533NIGMS NIH HHS R01 GM087221NIGMS NIH HHS R01 GM147947NIGMS NIH HHS R35 GM130293NIH HHS S10 OD026936
6 · The paper itself

Abstract

Liquid chromatography-tandem mass spectrometry employing data-dependent acquisition (DDA) is a mature, widely used proteomics technique routinely applied to proteome profiling, protein-protein interaction studies, biomarker discovery, and protein modification analysis. Numerous tools exist for searching DDA data and myriad file formats are output as results. While some search and post processing tools include data visualization features to aid biological interpretation, they are often limited or tied to specific software pipelines. This restricts the accessibility, sharing and interpretation of data, and hinders comparison of results between different software pipelines. We developed Limelight, an easy-to-use, open-source, freely available tool that provides data sharing, analysis and visualization and is not tied to any specific software pipeline. Limelight is a data visualization tool specifically designed to provide access to the whole "data stack", from raw and annotated scan data to peptide-spectrum matches, quality control, peptides, proteins, and modifications. Limelight is designed from the ground up for sharing and collaboration and to support data from any DDA workflow. We provide tools to import data from many widely used open-mass and closed-mass search software workflows. Limelight helps maximize the utility of data by providing an easy-to-use interface for finding and interpreting data, all using the native scores from respective workflows.

Indexed as

ProteomicsSoftwareTandem Mass SpectrometryChromatography, LiquidDatabases, ProteinData VisualizationHumansInformation DisseminationInternetMass Spectrometrydata visualizationDDAmass spectrometryproteomicsserversoftware development

Identifiers

PMID40036265
PMCPMC11977539

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.