ArticlePloS one2025
Expression of transgenic biotin ligases in inducible neuronal murine cell lines by integration into the mHipp11 gene locus.
Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biotin proximity labeling is a powerful method for identifying proteins associated with a specific organelle, a bait protein, or RNA. It requires the expression of a modified biotin ligase by transient transfection or from a stably integrated expression construct. Because such stable integration of transgenes into stem cells can lead to silencing during differentiation, targeting a biotin ligase to a genomic safe harbor site would be beneficial. Here, we report on the successful targeting and expression of two biotin ligase constructs to the mouse Hipp11 locus during neuronal differentiation. While randomly integrated MicroID and TurboID are expressed and active in mouse embryonic stem cells (mESCs), expression ceases upon differentiation into mESC-derived neurons, which is independent of the promoter used. In contrast, targeting of the same expression cassette to the mHipp11 locus results in expression, correct localization, and biotinylation activity not only in mESCs but also in neurons 8-10 days after differentiation. This demonstrates that the mouse Hipp11 locus is a promising genomic integration site for transgenic biotin ligases in mESCs and mESC-derived neurons.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.