Evidence map›Paper›PMID 40035494›Full record

ArticleActa crystallographica. Section F, Structural biology communications2025

Analysis of Burkholderia pseudomallei IspF in complex with sulfapyridine, sulfamonomethoxine, ethoxzolamide and acetazolamide.

Dakota Grote, Christopher G Stewart, Drashti G Daraji, Parisa Enayati, Kristina N Braverman, CeAnn Romanaggi, Madison J Bolejack, Jason K Yano, Jan Abendroth, David M Dranow and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Dakota GroteDepartment of Chemistry and Biochemistry, Northern Illinois University, 1425 Lincoln Highway, DeKalb, IL 60115, USA.
Christopher G StewartDepartment of Chemistry and Biochemistry, Northern Illinois University, 1425 Lincoln Highway, DeKalb, IL 60115, USA.
Drashti G DarajiDepartment of Chemistry and Biochemistry, Northern Illinois University, 1425 Lincoln Highway, DeKalb, IL 60115, USA.
Parisa EnayatiDepartment of Chemistry and Biochemistry, Northern Illinois University, 1425 Lincoln Highway, DeKalb, IL 60115, USA.ORCID 0009-0001-7500-6592
Kristina N BravermanSeattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98109, USA.
CeAnn RomanaggiSeattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98109, USA.
Madison J BolejackSeattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98109, USA.
Jason K YanoSeattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98109, USA.
Jan AbendrothSeattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98109, USA.ORCID 0000-0002-8229-3243
David M DranowSeattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98109, USA.
Phillip G PierceUCB Biosciences, Bainbridge Island, WA 98110, USA.ORCID 0000-0002-8176-770X
Donald D LorimerSeattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98109, USA.ORCID 0000-0002-9638-8083
Peter S HoranyiSeattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98109, USA.
Bart L StakerSeattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98109, USA.
Thomas E EdwardsSeattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98109, USA.ORCID 0000-0002-0474-8003
Peter J MylerSeattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98109, USA.ORCID 0000-0002-0056-0513
James R HornDepartment of Chemistry and Biochemistry, Northern Illinois University, 1425 Lincoln Highway, DeKalb, IL 60115, USA.ORCID 0000-0002-5694-0232
Timothy J HagenDepartment of Chemistry and Biochemistry, Northern Illinois University, 1425 Lincoln Highway, DeKalb, IL 60115, USA.ORCID 0000-0003-1929-9121

Funding

Centers for Research on Structural Biology of Infectious Diseases: Universal Influenza Vaccine Research75N93022C00036 · NIAID · SEATTLE CHILDREN'S HOSPITAL · PI STAKER, BART · 2022 to 2025
$21.7M
Design and Study of IspF Inhibitors as Antibacterial AgentsR15AI113653 · NIAID · NORTHERN ILLINOIS UNIVERSITY · PI HAGEN, TIMOTHY JOSEPH · 2014 to 2014
$356k
National Institutes of Health, National Institute of Allergy and Infectious Diseases 1R15AI113653-01National Science Foundation, Chemistry 1726931National Science Foundation, Chemistry CHE-2117776NIAID NIH HHS HHSN272200700057CNIAID NIH HHS HHSN272201200025CNIAID NIH HHS R15 AI113653NIH HHS 75N93022C00036NIH HHS HHSN272201700059C
6 · The paper itself

Abstract

The methylerythritol phosphate (MEP) pathway is a metabolic pathway that produces the isoprenoids isopentyl pyrophosphate and dimethylallyl pyrophosphate. Notably, the MEP pathway is present in bacteria and not in mammals, which makes the enzymes of the MEP pathway attractive targets for the discovery of new anti-infective agents due to the reduced chances of off-target interactions leading to side effects. There are seven enzymes in the MEP pathway, the fifth of which is IspF. Crystal structures of Burkholderia pseudomallei IspF were determined with five different sulfonamide ligands bound. The sulfonamide-containing ligands were ethoxzolamide, acetazolamide, sulfapyridine and sulfamonomethoxine. The fifth bound ligand was a synthetic analog of acetazolamide. All ligands coordinated to the active-site Zn

Indexed as

AcetazolamideBacterial ProteinsBurkholderia pseudomalleiEthoxzolamideSulfapyridineCatalytic DomainCrystallography, X-RayModels, MolecularProtein BindingSulfonamidesZincAcetazolamideBacterial ProteinsEthoxzolamideSulfapyridineSulfonamidesZinc2C-methyl-d-erythritol 2,4-cyclodiphosphate synthaseBurkholderia pseudomalleiIspFlyasesSeattle Structural Genomics Center for Infectious Diseasestructural genomics

Identifiers

PMID40035494
PMCPMC11970122

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.