Evidence map›Paper›PMID 40034450›Full record

ArticleResearch square2025

Field evaluation of the Bioline Malaria Ag P.f/Pan Rapid Diagnostic Test: Causes of Microscopy Discordance and Performance in Uganda.

Kisakye Diana Kabbale, Bienvenu Nsengimaana, Francis D Semakuba, Brian A Kagurusi, Caroline Mwubaha, Innocent Wiringilimaana, Thomas Katairo, Shahiid Kiyaga, Monica Mbabazi, Samuel Gonahasa and 15 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

25 authors.

Kisakye Diana KabbaleInfectious Diseases Research Collaboration.
Bienvenu NsengimaanaInfectious Diseases Research Collaboration.
Francis D SemakubaInfectious Diseases Research Collaboration.
Brian A KagurusiInfectious Diseases Research Collaboration.
Caroline MwubahaInfectious Diseases Research Collaboration.
Innocent WiringilimaanaInfectious Diseases Research Collaboration.
Thomas KatairoInfectious Diseases Research Collaboration.
Shahiid KiyagaInfectious Diseases Research Collaboration.
Monica MbabaziInfectious Diseases Research Collaboration.
Samuel GonahasaInfectious Diseases Research Collaboration.
Moses R KamyaInfectious Diseases Research Collaboration.
Stephen TukwasibweInfectious Diseases Research Collaboration.
Sam L NsobyaInfectious Diseases Research Collaboration.
Victor AsuaInfectious Diseases Research Collaboration.
Daudi JjingoAfrican Center of Excellence in Bioinformatics and Data Intensive Sciences, Makerere University.
Bosco AgabaMinistry of Health.
Catherine Maiteki-SebuguziMinistry of Health.
Jimmy OpigoMinistry of Health.
Kylie HiltonUniversity of California.
Sarah G StaedkeLiverpool School of Tropical Medicine.
Grant DorseyUniversity of California San Francisco.
Melissa D ConradUniversity of California San Francisco.
Bryan GreenhouseUniversity of California San Francisco.
Isaac SsewanyanaCentral Public Health Laboratories.
Jessica BriggsUniversity of California San Francisco.

Funding

Transmission ProjectU19AI089674 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MATTHEW G DORSEY, MOSES Robert KAMYA · 2010 to 2026
$29.3M
Mentoring translational scientists in international infectious disease researchK24AI144048 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Bryan R Greenhouse · 2019 to 2026
$1.6M
Improving the accuracy of malaria surveillance with serology and parasite genetic dataK23AI166009 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Jessica Briggs · 2022 to 2026
$955k
Gates Foundation INV-035751Gates Foundation INV-037316NIAID NIH HHS K23 AI166009NIAID NIH HHS K24 AI144048NIAID NIH HHS U19 AI089674
6 · The paper itself

Abstract

Background: Histidine Rich Protein 2 (HRP2)/pan-Lactate Dehydrogenase (pLDH) combination Rapid Diagnostic Tests (RDTs) may address the shortcomings of RDTs that detect HRP2 alone. However, the relative contribution of the possible causes of discordant results (RDT-negative and microscopy-positive) and performance in field settings are poorly quantified. Methods: This study utilized samples from two cross-sectional surveys conducted in 32 districts at 64 sites across Uganda between November 2021 and March 2023 that enrolled 6354 febrile participants ≥ two years of age. Discordant samples (negative by HRP2/pLDH RDT and positive by microscopy) underwent quantitative PCR (qPCR) to detect and quantify parasitemia. Those confirmed to be positive for Results: There were 166 (8.4%) discordant samples out of 1988 microscopy positive samples. Of these, 90/166 (54.2%) were confirmed to contain Conclusion: The Bioline Malaria Ag P.f/Pan combination RDT was found to be highly sensitive in Uganda and reliable for ruling out malaria. False negative RDT results were primarily due to low density

Indexed as

discordanceHRP2/pLDH combination Rapid Diagnostic TestMalariaperformancepfhrp2pfhrp3Plasmodium falciparumsensitivityspecificity

Identifiers

PMID40034450
PMCPMC11875311

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.