Evidence map›Paper›PMID 40034082›Full record

ArticleACS infectious diseases2025

Fluorescence Lifetime Imaging Detects Long-Lifetime Signal Associated with Reduced Pyocyanin at the Surface of

Tara Gallagher, Simon Leemans, Alexander S Dvornikov, Kumar Perinbam, Joshua Fong, Christina Kim, Joseph Kapcia, Miki Kagawa, Adam Grosvirt-Dramen, Allon I Hochbaum and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Tara GallagherUniversity of California Irvine, Department of Molecular Biology and Biochemistry, Irvine, California 92697-2525, United States.
Simon LeemansUniversity of California Irvine, Department of Biomedical Engineering, Irvine, California 92697-2715, United States.
Alexander S DvornikovUniversity of California Irvine, Department of Biomedical Engineering, Irvine, California 92697-2715, United States.
Kumar PerinbamUniversity of California Irvine, Department of Molecular Biology and Biochemistry, Irvine, California 92697-2525, United States.
Joshua FongUniversity of California Irvine, Department of Molecular Biology and Biochemistry, Irvine, California 92697-2525, United States.
Christina KimUniversity of California Irvine, Department of Molecular Biology and Biochemistry, Irvine, California 92697-2525, United States.
Joseph KapciaUniversity of California Irvine, Department of Molecular Biology and Biochemistry, Irvine, California 92697-2525, United States.
Miki KagawaUniversity of California Irvine, Department of Molecular Biology and Biochemistry, Irvine, California 92697-2525, United States.
Adam Grosvirt-DramenUniversity of California Irvine, Department of Chemical and Biomolecular Engineering, 6000 Interdisciplinary Science & Engineering Building (ISEB), 916 Engineering Tower, Irvine, California 92617-2580, United States.
Allon I HochbaumUniversity of California Irvine, Department of Molecular Biology and Biochemistry, Irvine, California 92697-2525, United States.ORCID 0000-0002-5377-8065
Michelle A DigmanUniversity of California Irvine, Department of Biomedical Engineering, Irvine, California 92697-2715, United States.ORCID 0000-0003-4611-7100
Enrico GrattonUniversity of California Irvine, Department of Biomedical Engineering, Irvine, California 92697-2715, United States.
Albert SiryapornUniversity of California Irvine, Department of Physics and Astronomy, Irvine, California 92697-4575, United States.
Katrine WhitesonUniversity of California Irvine, Department of Molecular Biology and Biochemistry, Irvine, California 92697-2525, United States.ORCID 0000-0002-5423-6014

Funding

Uncover Strategies Used By Biological SystemsP50GM076516 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI NIE, QING · 2007 to 2016
$25.1M
Dynamics of airway microbiota at pulmonary exacerbation in cystic fibrosisR01HL136647 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LIPUMA, JOHN J · 2017 to 2020
$1.3M
NHLBI NIH HHS R01 HL136647NIGMS NIH HHS P50 GM076516
6 · The paper itself

Abstract

Understanding bacterial physiology in real-world environments requires noninvasive approaches and is a challenging yet necessary endeavor to effectively treat infectious disease. Bacteria evolve strategies to tolerate chemical gradients associated with infections. The DIVER (Deep Imaging Via Enhanced Recovery) microscope can image autofluorescence and fluorescence lifetime throughout samples with high optical scattering, enabling the study of naturally formed chemical gradients throughout intact biofilms. Using the DIVER, a long fluorescent lifetime signal associated with reduced pyocyanin, a molecule for electron cycling in low oxygen, was detected in low-oxygen conditions at the surface of

Indexed as

BiofilmsOptical ImagingPseudomonas aeruginosaPyocyaninePyocyaninebiofilmFluorescencefluorescence lifetimephenazinesPseudomonas aeruginosapyocyanin

Identifiers

PMID40034082
PMCPMC11915379

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.