ArticleMolecular plant pathology2025
gma-miR828a Negatively Regulates Resistance to Tea Leaf Spot Caused by Lasiodiplodia theobromae Through Targeting the CsMYB28-CsRPP13 Module.
Article in Molecular plant pathology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Tea Plant miR5368-p5 Negatively Regulates Resistance Against Lasiodiplodia theobromae Through Targeting the CsWRKY57-CsLRR-RLK Module.Molecular plant pathology · 2025Article
- Uncovering the key miRNA-target network of tea plants in resistance to sooty mold disease.BMC plant biology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Leaf spot caused by the fungus Lasiodiplodia theobromae severely affects the quality and production of tea (Camellia sinensis) in plantations across southwestern China. Currently, no effective control measures are available, and the damage to tea leaves is also exacerbated by a lack of understanding regarding the epidemiology of the disease. Previous studies have suggested that gma-miR828a is differentially expressed during L. theobromae infection and may target and cleave the mRNA of CsMYB28. In this study, we characterised CsMYB28 as encoding a transcription factor (TF) that localises to the nucleus, cell membrane, and cytoplasm. This gene was found to be differentially and spatiotemporally expressed in leaf tissues following L. theobromae infection of leaves of the tea plant. Altered CsMYB28 expression, achieved by transient overexpression or stable genetic transformation of Nicotiana benthamiana, or transient silencing using antisense oligonucleotides (AsODN) in the tea plant, indicated that CsMYB28 contributes to resistance against L. theobromae. Using DNA affinity purification sequencing, yeast one-hybrid, and dual-luciferase assays, we also identified that CsMYB28 bound to the AATTAATT motif of CsRPP13, thereby activating the expression of CsRPP13. Additionally, degradome sequencing, β-glucuronidase (GUS) assays, and RNA ligase-mediated rapid amplification of cDNA ends revealed that miR828a cleaved CsMYB28 mRNA, negatively regulating its expression. The results from transient overexpression and stable transformation studies, combined with AsODN-mediated silencing in the tea plant, suggested that miR828a plays a negative regulatory role in modulating the response of the tea plant to L. theobromae infection. This study demonstrates that the miR828a-CsMYB28-CsRPP13 mediates the response of the tea plant to L. theobromae infection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.