Evidence map›Paper›PMID 40033327›Full record

ArticleJournal of animal science and biotechnology2025

Functional validation to explore the protective role of miR-223 in Staphylococcus aureus-induced bovine mastitis.

Xueqin Liu, Siyuan Mi, Gerile Dari, Siqian Chen, Jiuzhou Song, David E MacHugh, Ying Yu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xueqin LiuCollege of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Siyuan MiCollege of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Gerile DariCollege of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Siqian ChenCollege of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Jiuzhou SongDepartment of Animal and Avian Sciences, University of Maryland, College Park, MD, USA.
David E MacHughUCD School of Agriculture and Food Science, University College Dublin, Dublin, D04 V1W8, Ireland. david.machugh@ucd.ie.
Ying YuCollege of Animal Science and Technology, China Agricultural University, Beijing, 100193, China. yuying@cau.edu.cn.ORCID http://orcid.org/0000-0003-4772-413X

Funding

Beijing Dairy Industry Innovation Team BAIC06National Key Research and Development Program of China 2021YFD1200903National Key Research and Development Program of China 2023YFF1000902National Science Foundation for Young Scientists of China 32302706
6 · The paper itself

Abstract

backgroundMastitis caused by Staphylococcus aureus (S. aureus) is one of the most intractable problems for the dairy industry, causing significantly reduced milk yields and early slaughter of cows worldwide. MicroRNAs (miRNAs) can post-transcriptionally regulate gene expression and studies in recent years have shown the importance of miRNA-associated gene regulation in S. aureus-induced mastitis.

resultsIn this study, to investigate the role of miR-223 in mastitis, we performed experiments to overexpress and suppress miR-223 in an immortalized bovine mammary epithelial cell line (MAC-T) infected with S. aureus. Overexpression of miR-223 in MAC-T cells repressed cell apoptosis and necrosis induced by S. aureus infection, whereas suppression of miR-223 had the opposite effect. Transcriptome expression profiling with weighted gene co-expression network analysis (WGCNA) and gene set variation analysis (GSVA) showed that miR-223 affects apoptosis and inflammation-related pathways. Furthermore, differentially expressed (DE) genes were evaluated, and genes exhibiting contrasting expression trends in the miR-223 overexpressed and suppressed groups were assessed as potential target genes of miR-223. Potential target genes, including CDC25B, PTPRF, DCTN1, and DPP9, were observed to be associated with apoptosis and necroptosis. Finally, through integrative analysis of genome-wide association study (GWAS) data and the animal quantitative trait loci (QTL) database, we determined that target genes of miR-223 were significantly enriched in single-nucleotide polymorphisms (SNP) and QTLs related to somatic cell count (SCC) and mastitis.

conclusionIn summary, miR-223 has an inhibitory effect on S. aureus-induced cell apoptosis and necrosis by regulating PTPRF, DCTN1, and DPP9. These genes were significantly enriched in QTL regions associated with bovine mastitis resistance, underscoring their relevance in genetic regulation of disease resilience. Our findings provide critical genetic markers for enhancing mastitis resistance, particularly S. aureus-induced mastitis, through selective breeding. This work offers valuable insights for developing cattle with improved resistance to mastitis via targeted genetic selection.

Indexed as

Bovine mastitisGene regulationMammary epithelial cellsMiR-223Staphylococcus aureus

Identifiers

PMID40033327
PMCPMC11877765

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.