ArticleTropical animal health and production2025
Expression and characterization of the immunogenicity of rec-gp45 of Babesia bigemina using cattle.
Article in Tropical animal health and production, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Bovine Babesiosis (BB) is a tick-borne blood protozoan disease caused by Babesia, worldwide. The disease in tropical and subtropical regions is caused by B. bovis and B. bigemina. The main aim of this study was to develop the rec-gp45 of B. bigemina and to do experiments for the conduction of promising immunogenic trials on calves. Initially, microscopy was performed to identify B. bigemina through a thin blood smear, and DNA extraction was done. Subsequently, PCR was performed to amplify the gp45 sequence. Transformation of pET28α-gp45 into BL-21 was done by heat shock method. The rec-gp45 was identified at 39 kDa through SDS-PAGE and Western blot analyses. Indirect ELISA was performed to check specificity and sensitivity. Flow cytometry was also performed to assess the cellular responses following inoculation of rec-gp45 in calves. Indirect ELISA showed the specificity and sensitivity 100%, respectively. The immunogenic responses against BB in calves were checked through flow cytometry and we found 29.96% of CD8 + and 1.390% of CD4 + T cells, whereas cellular and humoral responses were shown against BB and it showed specific IgG response with OD value 2.460 ± 0.3151. The recombinant protein gp45 can be a promising immunogenic candidate against B. bigemina.
Indexed as
Identifiers
40032701What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.