Evidence map›Paper›PMID 40030016›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

Cas9-mediated gene-editing frequency in microalgae is doubled by harnessing the interaction between importin α and phytopathogenic NLSs.

Trang Thi Le, Hong Il Choi, Ji Won Kim, Jin-Ho Yun, Yoon Hyeok Lee, Eun Jung Jeon, Kil Koang Kwon, Dae-Hyun Cho, Dong-Yun Choi, Su-Bin Park and 5 more

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Harnessing microalgae for bioproducts: innovations in synthetic biology.World journal of microbiology & biotechnology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Trang Thi Le *Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.ORCID 0000-0003-2027-1628
Hong Il Choi *Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.ORCID 0000-0003-4475-4763
Ji Won Kim *Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.ORCID 0000-0002-9646-8374
Jin-Ho YunCell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.ORCID 0000-0002-0689-6739
Yoon Hyeok LeeDesign AI Lab, AI Center Samsung Electronics, Suwon-si, Gyeonggi-do 16678, South Korea.ORCID 0000-0002-3622-5266
Eun Jung JeonSynthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.ORCID 0000-0002-9314-7997
Kil Koang KwonSynthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.
Dae-Hyun ChoCell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.ORCID 0000-0001-8975-2468
Dong-Yun ChoiCell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.
Su-Bin ParkCell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.ORCID 0000-0003-2083-7150
Hyang Ran YoonImmunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.
Jeongmi LeeGenome Editing Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.
Eun Jeong SimCell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.
Yong Jae LeeCell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.ORCID 0000-0001-9917-8199
Hee-Sik KimCell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea.

Funding

National Research Foundation of Korea (NRF) 2021R1C1C1003425National Research Foundation of Korea (NRF) RS-2024-00459155
6 · The paper itself

Abstract

Pathogen-derived nuclear localization signals (NLSs) enable vigorous nuclear invasion in the host by the virulence proteins harboring them. Herein, inspired by the robust nuclear import mechanism, we show that NLSs originating from the plant infection-associated

Indexed as

alpha KaryopherinsChlamydomonas reinhardtiiCRISPR-Associated Protein 9CRISPR-Cas SystemsGene EditingMicroalgaeNuclear Localization SignalsAgrobacteriumBacterial ProteinsCell Nucleusalpha KaryopherinsBacterial ProteinsCRISPR-Associated Protein 9Nuclear Localization SignalsagrobacteriumCRISR/Cas9gene editing frequencymicroalgaenuclear localization signal (NLS)

Identifiers

PMID40030016
PMCPMC11912399

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.