ArticleJournal of the American Chemical Society2025
Fungal RiPPs Side Chain Macrocyclization Catalyzed by Copper-Dependent DUF3328 Enzyme.
Article in Journal of the American Chemical Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed.
- Emerging classes of copper enzymes.Current opinion in chemical biology · 2026Review
- P450 cyptide synthase KwwB catalyzes non-native cross-link at Tyr-C3-Trp-N1 and tolerates leader peptide mutations.The Journal of antibiotics · 2026Article
- P450 Cyptide Synthase AscB Catalyzes Fused Cyclophane at the YxWxH Motif on the Precursor Peptides.Organic letters · 2026Article
- When the wrong metal stops the cycle: Dynamics and specificity in plant copper-dependent peptide cyclases.Journal of inorganic biochemistry · 2026Article
- RadicalJournal of the American Chemical Society · 2026Article
- P450 Cyptide Synthase KwwB Catalyzes Trp-C5-Trp-N1 Cross-Linking and Accepts Diverse Precursor Peptides.Organic letters · 2026Article
- Leader-Independent C‑Terminal Modification by a RadicalACS bio & med chem Au · 2025Article
- Cyclochlorotine Hydroxylase CctR Reveals DUF3328 as a Family of Copper-Dependent Metalloenzymes.Angewandte Chemie (International ed. in English) · 2025Article
- Copper-Dependent Hydroxylation Catalyzed by the DUF3328 Enzyme CctR.Organic letters · 2025Article
- Peptide Recognition Sequence Guides Catalytic Side Chain Cross-Linking of Plant Peptides by Copper-Dependent Cyclases.Journal of the American Chemical Society · 2025Article
- P450 Biarylitide Synthase ShyB Catalyzes the Cross-Link Between His-C2 and Tyr-O4 on the Precursor Peptide.Organic letters · 2025Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Fungal enzymes containing domain of unknown function (DUF) 3328 have been implicated in the oxidative maturation of ribosomally synthesized and post-translationally modified peptides (RiPPs). We report here the functional characterization of one such enzyme, AprY, involved in the biosynthesis of RiPP asperipin-2a. Biochemical reconstitution of AprY showed the enzyme catalyzes two consecutive C-O cross-linking reactions between tyrosines and beta-carbons of residues in the core hexapeptide. The side-chain macrocyclization activities of AprY are copper- and oxygen-dependent and do not require a leader peptide sequence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.