ArticlePlant biotechnology journal2025
Combined enhancement of ascorbic acid, β-carotene and zeaxanthin in gene-edited lettuce.
Article in Plant biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Molecular Targets and Trait Innovation in CRISPR-Edited Vegetable Crops: An Up-to-Date Review (2020-2026).Plants (Basel, Switzerland) · 2026Review
- Reconfiguring biofortification strategies to transform food systems and address micronutrient deficiency of the 21st century.Journal of integrative plant biology · 2026Review
- Review
- Gene-Editing-Mediated Enhancement of Carotenoid Compound Accumulation in Common Wheat Grains.Foods (Basel, Switzerland) · 2026Article
- Targeted Gene Modification ofInternational journal of molecular sciences · 2026Article
- Modern approaches and future prospects in lettuce breeding for controlled environment agriculture.Frontiers in plant science · 2026Review
- CRISPR/Cas9-mediated knockout ofFrontiers in genome editing · 2026Article
- Article
- Plant synthetic biology-based biofortification, strategies and recent progresses.Journal of integrative plant biology · 2025Review
- Development of an endogenous promoter-driven CRISPR/Cas9 system for genome editing inForestry research · 2025Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Lettuce is widely grown and consumed but provides lower nutritional value compared to other leafy greens, particularly in the essential vitamins A and C. To address this, major control points in carotenoid and ascorbic acid (AsA) production were targeted using a viral-based CRISPR/Cas9 system in the commercial lettuce cultivar 'Noga'. Knockout of lycopene ε-cyclase (LCY-ε), the enzymatic gatekeeper opposing production of β-branch carotenoids, increased β-carotene (provitamin A) levels up to 2.7-fold and facilitated zeaxanthin accumulation up to 4.3 μg/g fresh weight. Chlorophyll fluorescence measurements revealed that photosystem II efficiency was unaffected in LCY-ε mutants, though their non-photochemical quenching (NPQ) capacity decreased at light intensities above 400 μmol m
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Registered trials
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