Evidence map›Paper›PMID 40029526›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

A Comprehensive Methodology for Immortalizing Tumor-Infiltrating B Lymphocytes from Epithelial Cancers.

Baishali Tamuli, Subir Biswas

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Baishali TamuliCancer Immune Environment and Therapeutics Lab, Tumor Immunology and Immunotherapy, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, India.
Subir BiswasCancer Immune Environment and Therapeutics Lab, Tumor Immunology and Immunotherapy, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, India. sbiswas@actrec.gov.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Epithelial cancers are often infiltrated with prognostically relevant lymphocytes, including T and B lymphocytes. Isolation, expansion, and immortalization of lymphocytes from epithelial tumors are immensely important in basic tumor immunology research and clinical translation. Here, we outline in detail the process of separating viable B lymphocytes from surgically removed epithelial tumors and their immortalization and cryopreservation methods. Overall, this state-of-the-art procedure aids in bulk and specific antigen-reactive antibody production from tumor-infiltrating B cells. Also, sequence information can be deduced by cutting-edge sequencing methods from these immortalized pools of B lymphocytes.

Indexed as

B-LymphocytesLymphocytes, Tumor-InfiltratingCell SeparationCryopreservationHumansAntitumor responseEpithelial cancersEpstein–Barr virusImmortalizationTertiary lymphoid structuresTumor-infiltrating B lymphocytes

Identifiers

PMID40029526

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.