ArticleMethods in molecular biology (Clifton, N.J.)2025
Immunophenotyping of B-Cell Subpopulations by Flow Cytometry.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This chapter introduces a protocol for immunophenotyping B-cell subsets using flow cytometry, a vital technique in both immunology research and clinical practice. Flow cytometry offers detailed insights into B-cell diversity and function, aiding in understanding B-cell development and guiding therapeutic decisions. This chapter delves into critical aspects such as antibody panels, sample preparation, and data analysis, all of which are essential for effectively analyzing B-cell subpopulations in diagnosing conditions like common variable immunodeficiency and monitoring therapeutic outcomes after B-cell depletion. The described protocol ensures accurate quantitative and qualitative determination of total B cells and subpopulations, providing a valuable resource for researchers and clinicians alike.
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