Evidence map›Paper›PMID 40028290›Full record

ArticleFrontiers in bioengineering and biotechnology2025

Intensification of Vero cell adherence to microcarrier particles during cultivation in a wave bioreactor.

Z K Mazhed, V E Vasilenko, A A Siniugina, K V Kaa, A S Motov, K O Pokidova, Y Y Ivin, A N Piniaeva, Y K Khapchaev, K A Chernov and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Z K MazhedFederal State Autonomous Scientific Institution "Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences" (FSASI "Chumakov FSC R&D IBP RAS"), Moscow, Russia.
V E VasilenkoFederal State Autonomous Scientific Institution "Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences" (FSASI "Chumakov FSC R&D IBP RAS"), Moscow, Russia.
A A SiniuginaFederal State Autonomous Scientific Institution "Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences" (FSASI "Chumakov FSC R&D IBP RAS"), Moscow, Russia.
K V KaaFederal State Autonomous Scientific Institution "Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences" (FSASI "Chumakov FSC R&D IBP RAS"), Moscow, Russia.
A S MotovFederal State Autonomous Scientific Institution "Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences" (FSASI "Chumakov FSC R&D IBP RAS"), Moscow, Russia.
K O PokidovaFederal State Autonomous Scientific Institution "Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences" (FSASI "Chumakov FSC R&D IBP RAS"), Moscow, Russia.
Y Y IvinFederal State Autonomous Scientific Institution "Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences" (FSASI "Chumakov FSC R&D IBP RAS"), Moscow, Russia.
A N PiniaevaFederal State Autonomous Scientific Institution "Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences" (FSASI "Chumakov FSC R&D IBP RAS"), Moscow, Russia.
Y K KhapchaevFederal State Autonomous Scientific Institution "Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences" (FSASI "Chumakov FSC R&D IBP RAS"), Moscow, Russia.
K A ChernovFederal State Autonomous Scientific Institution "Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences" (FSASI "Chumakov FSC R&D IBP RAS"), Moscow, Russia.
A A IshmukhametovFederal State Autonomous Scientific Institution "Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products of the Russian Academy of Sciences" (FSASI "Chumakov FSC R&D IBP RAS"), Moscow, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vaccination is the most effective strategy for fighting viral diseases, with both live and inactivated vaccines remaining crucial despite advancements in subunit vaccine technologies. A key player in vaccine production is the Vero cell line, derived from the kidney cells of the African green monkey, which is essential for manufacturing vaccines against diseases like polio, rabies, yellow fever, and COVID-19. The efficiency of Vero cell cultivation directly impacts vaccine production, often utilizing bioreactors ranging from small (1-10 L) to large (up to several thousand liters). Wave-type bioreactors are commonly employed for initial cell propagation due to their simplicity. However, achieving uniform cell distribution on microcarriers in these systems poses challenges. This study aims to evaluate intermittent stirring during the early cultivation stages to enhance Vero cell distribution and growth, potentially improving overall cultivation efficiency.

Indexed as

bioreactor technologycell cultivationcytodex-1microcarrier particlesupstream process developmentvaccine productionVero cellswave bioreactor

Identifiers

PMID40028290
PMCPMC11868258

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.