Evidence map›Paper›PMID 40027990›Full record

ArticleRegenerative therapy2025

Subcutaneous implantation of tooth germ stem cells over the masseter muscle in mice: An in vivo pilot study.

Nosrat Nourbakhsh, Ghazaleh Baniebrahimi, Sahar Talebi, Ardeshir Talebi, Mohammad Hossein Nasr Esfahani, Bijan Movahedian, Maziar Manshayi, Navid Naghdi, Fatemeh Ejeian, Elahe Masaeli and 1 more

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Article in Regenerative therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Nosrat NourbakhshDepartment of Pediatric Dentistry, Dental Research Center, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Ghazaleh BaniebrahimiDepartment of Pediatric Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Sahar TalebiResearch Committee, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Ardeshir TalebiDepartment of Pathology, Medical School, Dental Research Center, Dental Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
Mohammad Hossein Nasr EsfahaniDepartment of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Bijan MovahedianDepartment of Maxillofacial Surgery, Dental Research Center, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Maziar ManshayiDental Science Research Center, Dentistry Faculty, Isfahan University of Medical Sciences, Isfahan, Iran.
Navid NaghdiDepartment of Maxillofacial Surgery, School of Dentistry, Hamedan University of Medical Sciences, Hamedan, Iran.
Fatemeh EjeianDepartment of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Elahe MasaeliDepartment of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Seyed Ali MosaddadDepartment of Research Analytics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objectives: This study aimed to explore the potential of tooth germ stem cells for regenerating tooth-like structures by subcutaneously implanting first molar tooth germ stem cells over the masseter muscle in mice. Methods: Five pairs of house mice, Results: H & E staining showed the formation of integrated oval structures at the implant site in all samples. Masson's trichrome staining identified dispersed accumulations of cellular mineralized matrix within the connective tissue. IHC staining was positive for vimentin, confirming the mesenchymal origin of the loose tissue at the center, indicating future dental pulp development. Positive CK staining indicated the ectodermal origin of dense peripheral tissues, suggesting the future formation of inner enamel epithelium. The combined immunohistochemical results for vimentin and CK confirmed the ecto-mesenchymal origin of the regenerated tissue, which resembled a late bell-stage tooth germ observed around gestational days 17.5-18 and showed early indications of dentin formation (D0). Conclusion: The study indicates that tooth germ stem cells may have the potential to produce dense, tooth-like structures when implanted subcutaneously in mice. These findings provide preliminary insights into the possible applications of tooth germ stem cells in regenerative dental tissue engineering.

Indexed as

Dental stem cellsImplantationStem cellsTissue engineeringTooth developmentTooth germTooth structure

Identifiers

PMID40027990
PMCPMC11869380

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