ArticleInfection and drug resistance2025
Evaluating a Dual-Gene qPCR Melting Curve Assay for Rapid Detection of Tuberculosis in Suspected Pulmonary Cases.
Article in Infection and drug resistance, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.
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Who cites it
4 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Diagnostic accuracy of stool Xpert MTB/RIF assay for adult pulmonary tuberculosis: a systematic review and meta-analysis.BMC infectious diseases · 2026Pooled it
- Salivary exosomalFrontiers in cellular and infection microbiology · 2026Article
- Article
- Biomarkers of pulmonary fibrosis in bronchoalveolar lavage fluid and saliva. Study methods and pathophysiological significance.Frontiers in medicine · 2026Review
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5 authors.
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Abstract
Purpose: New economic, rapid, and efficient diagnostic methods are desirable for the control of tuberculosis. This study aimed to evaluate the performance of a dual-gene qPCR melting curve assay (DGPMC) in detecting tuberculosis among patients with suspected pulmonary tuberculosis. Patients and Methods: The DGPMC assay based on Results: Overall, 240 patients were included in this trial, including 80 (33.3%) asymptomatic patients. Clinical diagnosis of tuberculosis was confirmed in 191 (79.6%) patients and 49 (20.4%) patients were confirmed without tuberculosis. The overall sensitivity of the DGPMC assay was 55.0% (95% CI: 47.6-62.1%), and the corresponding specificity was 85.7% (95% CI: 72.1-93.6%) in the diagnosis of tuberculosis. The sensitivity of DGPMC assay was higher than that of GeneXpert test (55.0% vs 47.1%, P = 0.038). The Youden index and weighted Youden index of the DGPMC assay were 40.7% and 28.4%, respectively. Subgroup analyses demonstrated that the sensitivity was 32.4% (95% CI: 22.3-44.4%) in the individuals with negative results for both culture and GeneXpert test. The DGPMC assay performed significantly better than the melting curves based on Conclusion: The DGPMC assay is an alternative tool favorable for the detection of tuberculosis in patients with suspected pulmonary tuberculosis, especially in the patients with low bacterial load.
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