Evidence map›Paper›PMID 40027778›Full record

ArticlebioRxiv : the preprint server for biology2025

A protein complex in the extreme distal tip of vertebrate motile cilia controls their organization, length, and function.

Juyeon Hong, Chanjae Lee, Gopika Madhu, Ophelia Papoulas, Ece Atayeter, Garbiel Hoogerbrugge, Jiehong Pan, Maki Takagishi, Nadia Manzi, Daniel J Dickinson and 6 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Juyeon HongDepartment of Molecular Biosciences, University of Texas at Austin, Texas, USA.
Chanjae LeeDepartment of Molecular Biosciences, University of Texas at Austin, Texas, USA.
Gopika MadhuDepartment of Physics, College of Arts and Sciences, University of Miami, Coral Gables, FL 33146, USA.
Ophelia PapoulasDepartment of Molecular Biosciences, University of Texas at Austin, Texas, USA.
Ece AtayeterDepartment of Molecular Biosciences, University of Texas at Austin, Texas, USA.
Garbiel HoogerbruggeDepartment of Molecular Biosciences, University of Texas at Austin, Texas, USA.
Jiehong PanDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Washington University School of Medicine in St. Louis, USA.
Maki TakagishiDept. of Medicinal and Life Sciences, Nagoya City University, Nagoya, Japan.
Nadia ManziDepartment of Molecular Biosciences, University of Texas at Austin, Texas, USA.
Daniel J DickinsonDepartment of Molecular Biosciences, University of Texas at Austin, Texas, USA.ORCID 0000-0003-2651-2584
Amjad HoraniDepartment of Pediatrics, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Steven L BrodyDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Washington University School of Medicine in St. Louis, USA.
Edward MarcotteDepartment of Molecular Biosciences, University of Texas at Austin, Texas, USA.
Vivek N PrakashDepartment of Physics, College of Arts and Sciences, University of Miami, Coral Gables, FL 33146, USA.ORCID 0000-0003-4569-6462
Tae Joo ParkDepartment of Biological Sciences, Ulsan National Institute of Science and Technology, Ulsan, South Korea.
John B WallingfordDepartment of Molecular Biosciences, University of Texas at Austin, Texas, USA.

Funding

REGULATION OF MOTILE CILIA ASSEMBLY IN LUNG DISEASER01HL128370 · NHLBI · WASHINGTON UNIVERSITY · PI Steven Brody, SUSAN K DUTCHER · 2015 to 2026
$6.9M
Proteomics and model organism humanization to decode human geneticsR35GM122480 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI EDWARD M MARCOTTE · 2017 to 2026
$5.4M
Development and function in mucociliary epitheliaR01HL117164 · NHLBI · UNIVERSITY OF TEXAS AT AUSTIN · PI John B Wallingford · 2013 to 2026
$4.7M
Cellular and Molecular Features of Gene Mutations in Primary Ciliary DyskinesiaR01HL146601 · NHLBI · WASHINGTON UNIVERSITY · PI BRODY, STEVEN · 2019 to 2022
$2.3M
NRF2 activation program in normal and immotile ciliaR01HL173490 · NHLBI · WASHINGTON UNIVERSITY · PI Amjad Horani · 2024 to 2026
$1.8M
NHLBI NIH HHS R01 HL117164NHLBI NIH HHS R01 HL128370NHLBI NIH HHS R01 HL146601NHLBI NIH HHS R01 HL173490NIGMS NIH HHS R35 GM122480
6 · The paper itself

Abstract

The beating of cilia on multi-ciliated cells (MCCs) is essential for normal development and homeostasis in animals. But while the structure and function of basal bodies and axonemes have received significant attention recently, the distal tips of MCC cilia remain relatively poorly defined. Here, we characterize the molecular organization of the distal tip of vertebrate MCC cilia, characterizing two distinct domains occupied by distinct protein constituents. Using frog, mouse, and human MCCs, we find that two largely uncharacterized proteins, Ccdc78 and Ccdc33 occupy a previously undefined region at the extreme distal tip, and these are required for the normal organization of all other known tip proteins. Ccdc78 and Ccdc33 each display robust microtubule-bundling activity both

Identifiers

PMID40027778
PMCPMC11870508

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.