Evidence map›Paper›PMID 40026575›Full record

ArticlemLife2025

Developing a microfluidic-based epicPCR reveals diverse potential hosts of the

Wenli Shen, Danrui Wang, Jiangtao Li, Yue Liu, Yinzhao Wang, Xingsheng Yang, Xi Peng, Bingliang Xie, Lei Su, Ziyan Wei and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Applied and environmental microbiology · 2026
    Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Wenli ShenCAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China.
Danrui WangCAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China.
Jiangtao LiState Key Laboratory of Marine Geology Tongji University Shanghai China.
Yue LiuCollege of Environmental Science and Engineering Liaoning Technical University Fuxin China.
Yinzhao WangMicrobiology Division, School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai China.ORCID 0000-0001-8715-1386
Xingsheng YangCAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China.
Xi PengCAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China.
Bingliang XieState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing China.
Lei SuState Key Laboratory of Marine Geology Tongji University Shanghai China.
Ziyan WeiState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing China.
Qing HeCAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China.
Zhiyi WangState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing China.
Kai FengCAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China.
Wenbin DuState Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing China.ORCID 0000-0002-7401-1410
Ye DengCAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China.ORCID 0000-0002-7584-0632

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Anaerobic methanotrophic (ANME) microbes play a crucial role in the bioprocess of anaerobic oxidation of methane (AOM). However, due to their unculturable status, their diversity is poorly understood. In this study, we established a microfluidics-based epicPCR (Emulsion, Paired Isolation, and Concatenation PCR) to fuse the 16S rRNA gene and

Indexed as

anaerobic oxidation of methanecold seepepicPCRmcrA gene

Identifiers

PMID40026575
PMCPMC11868836

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.