Evidence map›Paper›PMID 40026390›Full record

ArticleFrontiers in plant science2025

Validation of suitable reference microRNAs for qRT-PCR in

Yingting Zhang, Qingyu Yan, Hui Xia, Jie Yang, Xiangling Zeng, Zeqing Li, Xuan Cai, Jingjing Zou, Hongguo Chen

Abstract read
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In one paragraph

Article in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yingting ZhangNational Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China.
Qingyu YanNational Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China.
Hui XiaCollege of Forestry, Central South University of Forestry and Technology, Changsha, China.
Jie YangNational Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China.
Xiangling ZengNational Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China.
Zeqing LiNational Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China.
Xuan CaiNational Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China.
Jingjing ZouNational Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China.
Hongguo ChenNational Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans, Hubei University of Science and Technology, Xianning, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Sweet osmanthus ( Methods: In this study, qRT-PCR technology was combined with four algorithms (i.e., delta-Ct, geNorm, NormFinder, and BestKeeper) to systematically evaluate the expression stability of 14 candidate miRNAs across eleven environmental conditions, including under abiotic stress, under hormone and metal ion treatments, during flower opening and senescence, and across various tissues. Results: The results revealed that under hormone treatments, ofr-miR159b-3p, novel8, and novel3 exhibited high expression stability; under abiotic stress, ofr-miR159b-3p, novel8, ofr-miR403-3p, and novel2 demonstrated considerable stability; during metal ion treatments, novel3, ofr-miR159b-3p, novel33, novel2, and ofr-miR395e were identified as stable miRNAs; in different tissues, novel2 and ofr-miR395e were relatively stable; and during flower opening and senescence, novel33 and ofr-miR395e maintained stable expression. Discussion: This study represents the first comprehensive assessment of reference miRNA stability in

Indexed as

abiotic stresshormone treatmentsmetal ion treatmentsOsmanthus fragransreference miRNAs

Identifiers

PMID40026390
PMCPMC11868269

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.