ArticleFrontiers in oral health2025
Higher Reactive Oxygen Species and cellular aging in oral mucosal cells of young smokers: a comparative analytical study.
Article in Frontiers in oral health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Polymorphisms ADH1B rs1229984 and NAA25 rs4767364 correlate with head and neck cancer risk in Southern Punjab, Pakistan: a case-control study.Annals of medicine and surgery (2012) · 2026Article
- Salivary Oxidative Stress and Antioxidant Markers in Oral Leukoplakia: A Systematic Review and Meta-Analysis.Antioxidants (Basel, Switzerland) · 2026Review
- Change of oral microbiome diversity by smoking across different age groups.Frontiers in microbiology · 2025Article
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Authors and funding
7 authors.
Funding
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Abstract
Background: Cigarette smoke contains chemical components that cause excessive production of Reactive Oxygen Species (ROS), altering cell physiology and affecting key pathways. This leads to hyperinflammation, DNA damage, and cellular aging, which may cause oral and pulmonary pathologies. Our study aims to investigate the impact of smoking on ROS levels and cellular aging in oral mucosal cells. We compared Reactive oxygen Species and cellular aging between smokers and non-smokers. Secondarily, we also compared the results between young and old smokers. Methods: Oral swabs were taken from 50 smokers and 50 nonsmokers using a cytology brush. We quantified the reactive oxygen species (ROS) by using oxidized 2'7' dichlorodihydrofluorescein-diacetate (DCFH-DA) dye. To assess cellular aging, mRNA levels of the CYR61 gene-a cellular aging marker, were compared through RT-PCR. Results: It was found that smokers had a higher percentage of ROS in comparison to non-smokers ( Conclusion: It has been observed that smokers have a higher amount of ROS production and cellular aging in their oral mucosal cells. In young smokers, ROS and cellular aging were found to be higher compared to older smokers. This is quite concerning and could be a major factor leading to oral pathologies in smokers.
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