Evidence map›Paper›PMID 40025574›Full record

ArticleStem cell research & therapy2025

Generation of phenotypically stable and functionally mature human bone marrow MSCs derived Schwann cells via the induction of human iPSCs-derived sensory neurons.

Yu Pan, Haohui Lin, Manhon Chung, Yi Yang, Li Zhang, Xiaohua Pan, Sa Cai

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Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yu Pan *Laboratory of Regenerative Medicine, Medical School, The 2nd Affiliated Hospital of Shenzhen University, Shenzhen University, Shenzhen, China.
Haohui Lin *Laboratory of Regenerative Medicine, Medical School, The 2nd Affiliated Hospital of Shenzhen University, Shenzhen University, Shenzhen, China.
Manhon Chung *Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Yi YangLaboratory of Regenerative Medicine, Medical School, The 2nd Affiliated Hospital of Shenzhen University, Shenzhen University, Shenzhen, China.
Li ZhangLaboratory of Regenerative Medicine, Medical School, The 2nd Affiliated Hospital of Shenzhen University, Shenzhen University, Shenzhen, China.
Xiaohua PanLaboratory of Regenerative Medicine, Medical School, The 2nd Affiliated Hospital of Shenzhen University, Shenzhen University, Shenzhen, China.
Sa CaiLaboratory of Regenerative Medicine, Medical School, The 2nd Affiliated Hospital of Shenzhen University, Shenzhen University, Shenzhen, China. caisa@szu.edu.cn.ORCID http://orcid.org/0000-0002-1370-0028

Funding

National Natural Science Foundation of China 81272080National Natural Science Foundation of China 82072163Natural Science Foundation of Guangdong Province 2016A030313797
6 · The paper itself

Abstract

backgroundPhenotypically unstable Schwann cell-like cells (SCLCs), derived from mesenchymal stem cells (MSCs) require intercellular contact-mediated cues for Schwann cell (SCs)-fate commitment. Although rat dorsal root ganglion (DRG) neurons provide contact-mediated signals for the conversion of SCLCs into fate-committed SCs, the use of animal cells is clinically unacceptable. To overcome this problem, we previously acquired human induced pluripotent stem cell-derived sensory neurons (hiPSC-dSNs) as surrogates of rat DRG neurons that committed rat bone marrow SCLCs to the SC fate. In this study, we explored whether hiPSC-dSNs could mimic rat DRG neuron effects to obtain fate-committed SCs from hBMSC-derived SCLCs.

methodshiPSCs were induced into hiPSC-dSNs using a specific chemical small molecule combination. hBMSCs were induced into hBMSC-derived SCLCs in a specific culture medium and then co-cultured with hiPSC-dSNs to generate SCs. The identity of hBMSC-derived SCs (hBMSC-dSCs) was examined by immunofluorescence, western bolt, electronic microscopy, and RNA-seq. Immunofluorescence was also used to detect the myelination capacity. Enzyme-linked immunosorbent assay and neurite outgrowth analysis were used to test the secretion of neurotrophic factors.

resultsThe hBMSC-dSCs exhibited bi-/tri-polar morphology of SCs and maintained the expression of the SC markers S100, p75NTR, p0, GFAP, and Sox10, even after withdrawing the glia-inducing factors or hiPSC-dSNs. Electronic microscopy and RNA-seq analysis provided evidence that hBMSC-dSCs were similar to the original human SCs in terms of their function and a variety of characteristics. Furthermore, these cells formed MBP-positive segments and secreted neurotrophic factors to facilitate the neurite outgrowth of Neuro2A.

conclusionsThese results demonstrated that phenotypically stable and functionally mature hBMSC-dSCs were generated efficiently via the co-culture of hiPSC-dSNs and hBMSC-derived SCLCs. Our findings may provide a promising protocol through which stable and fully developed hBMSC-dSCs can be used for transplantation to regenerate myelin sheath.

Indexed as

Bone Marrow CellsInduced Pluripotent Stem CellsMesenchymal Stem CellsSchwann CellsSensory Receptor CellsAnimalsCell DifferentiationCells, CulturedCoculture TechniquesGanglia, SpinalHumansPhenotypeRatsFate commitmentHuman bone marrow mesenchymal stem cellsHuman induced pluripotent stem cellsMyelinationSchwann cellsSensory neurons

Identifiers

PMID40025574
PMCPMC11872329

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.