Evidence map›Paper›PMID 40025541›Full record

ArticleBMC veterinary research2025

Identification of interferon-stimulated response elements (ISREs) in canines.

Zhichao Pan, Yutong Liu, Huilin Dai, Siqi Xu, Miaomiao Qin, Ke Wang, Chenying Luo, Changqi Luo, Qinying Zhang, Zhaoping Liang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Zhichao Pan *College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Yutong Liu *College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Huilin DaiCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Siqi XuCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Miaomiao QinCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Ke WangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Chenying LuoCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Changqi LuoCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Qinying ZhangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Zhaoping LiangCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China. liangzp1017@126.com.
Saixiang FengCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, China. fengsx@scau.edu.cn.

Funding

National Key Research and Development Program of China 2022YFD1801003
6 · The paper itself

Abstract

Interferon (IFN) responses are vital for antiviral defense, with interferon-stimulated response elements (ISREs) crucial for regulating IFN signaling. While ISREs are well-studied in humans and mice, research on canine ISREs is limited. This study aimed to clarify the role of canine ISREs and create a new method for detecting IFN activity. Canine IFN α (CaIFNα) was produced using the Pichia pastoris (P. pastoris) system, and an ISRE-based flow cytometry method was developed to measure its activity. ISREs for CaIFNα were predicted via bioinformatics analysis. Subsequently, viral suppression assays were conducted using vesicular stomatitis virus, canine influenza virus, and H9N2 to evaluate the antiviral activity of recombinant CaIFNα. Fluorescence analysis confirmed that CaIFNα activates ISRE2, ISRE8, and ISRE10, thereby enhancing the transcription and expression of the enhanced green fluorescent protein (EGFP) fusion gene. A novel ISRE and EGFP based flow cytometry method enabled precise quantification of CaIFNα levels through fluorescence cell counts, with a detection sensitivity reaching 0.1 × 10

Indexed as

Interferon-alphaResponse ElementsAnimalsAntiviral AgentsDogsFlow CytometrySaccharomycetalesAntiviral AgentsInterferon-alphaAntiviral activityCanine interferon alphaEGFPFlow cytometryFluorescent reporter assayISRE

Identifiers

PMID40025541
PMCPMC11871823

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.