ArticleScientific reports2025
Lossless single-molecule counting to absolute quantify proteoforms.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors.
Funding
Abstract
A novel immunoassay, termed Protein Interaction Coupling (PICO), is introduced to deliver clear, reference-free quantification of proteoforms - precise quantification. PICO employs a compartmentalized, homogeneous single-molecule assay with lossless and sensitive signal generation, capable of detecting down to a few molecules per reaction. Additionally, it utilizes a background-free, digital enumeration principle, known as decouplexing. PICO is presented as mathematical theories, providing a theoretical understanding of its chemistry. Consequently, PICO demonstrates precise quantification, as exemplified with recombinant and non-recombinant ErbB2 and multi-tagged peptide rTRX targets, validating quantification against internal and external references in both analytical and cellular matrices. Furthermore, PICO enables combinatorial multiplexing (cplex), a readout between any two antibodies, demonstrated by an 8-plex antibody, 12-cplex PICO, measuring functional changes of the ErbB pathway upon mock and dactolisib treatment delivering quantitative cellular stoichiometry. PICO possesses significant potential for versatile, standardized, and accurate protein measurements, offering insights into physiological and perturbed cellular processes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.