Evidence map›Paper›PMID 40024238›Full record

ArticleJournal of musculoskeletal & neuronal interactions2025

Preliminary Exploration of the Osteogenic Differentiation Mechanism of Bone Marrow Mesenchymal Stem Cells Regulated by SYVN1.

Zhimin Pan, Zheng Wang, Yuanguo Zhou, Xinran Ji, Lei Yu, Xin Yin, Kai Song, Ning Yu, Yoon Ha, Nan Li and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of musculoskeletal & neuronal interactions, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Zhimin Pan *Department of Orthopaedics, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Jiangxi, China.
Zheng Wang *Department of Orthopedics, the Fourth Medical Center, Chinese PLA General Hospital, Beijing, China.
Yuanguo Zhou *Department of Orthopaedics, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Jiangxi, China.
Xinran Ji *Department of Orthopedics, the Fourth Medical Center, Chinese PLA General Hospital, Beijing, China.
Lei YuDepartment of Orthopaedics, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Jiangxi, China.
Xin YinDepartment of Orthopedics, the Fourth Medical Center, Chinese PLA General Hospital, Beijing, China.
Kai SongDepartment of Orthopedics, the Fourth Medical Center, Chinese PLA General Hospital, Beijing, China.
Ning YuDepartment of Orthopedics, the Fourth Medical Center, Chinese PLA General Hospital, Beijing, China.
Yoon HaDepartment of Neurosurgery, Spine and Spinal Cord Institute, Yonsei University College of Medicine, Seoul, Republic of Korea.
Nan LiDepartment of Spine Surgery, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.
Xing ZhuDepartment of Orthopaedics, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Jiangxi, China.
Yangbin WangDepartment of Orthopaedics, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Jiangxi, China.
Jiang HuangDepartment of Orthopaedics, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Jiangxi, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThe osteogenic differentiation ability of bone marrow mesenchymal stem cells (BMSCs) is an important aspect of studying osteoporosis (OP). This study aims to explore the role of SYVN1 in regulating the osteogenic differentiation of BMSCs and to suggest its potential as a treatment for OP.

methodsBMSCs were differentiated using osteogenic induction. The expression of SYVN1 at different osteogenic induction time points was analyzed by Western blot (WB). The expression levels of osteogenic markers, including RUNX2, ALP, and OCN, were measured by RT-qPCR. EdU staining and colony formation assays were performed to evaluate the impact of SYVN1 on the proliferative ability of BMSCs. The effect of SYVN1 on osteogenic differentiation of BMSCs was assessed by alizarin red staining. The association of SYVN1 with the AMPK/mTOR pathway was confirmed through WB analysis.

resultsThe expression of SYVN1 decreased as BMSCs differentiation progressed. Overexpression of SYVN1 inhibited the osteogenic differentiation and proliferation of BMSCs, whereas silencing SYVN1 had the opposite effect. Furthermore, SYVN1 overexpression reduced the p-AMPK/AMPK ratio and increased the p-mTOR/mTOR ratio, effects that were reversed by the AMPK activator A-769662.

conclusionSYVN1 overexpression inhibits the osteogenic differentiation and proliferation of BMSCs, potentially through modulation of the AMPK/mTOR pathway.

Indexed as

Bone Marrow CellsCell DifferentiationMesenchymal Stem CellsOsteogenesisAnimalsCell ProliferationCells, CulturedRatsRats, Sprague-DawleyAMPK/mTOR PathwayBMSCsOsteogenic DifferentiationSYVN1

Identifiers

PMID40024238
PMCPMC11880844

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.