Evidence map›Paper›PMID 40023843›Full record

ArticleCell reports2025

Temporal dynamics of immune cell transcriptomics in brain metastasis progression influenced by gut microbiome dysbiosis.

Samantha M Golomb, Ian H Guldner, Emilija Aleksandrovic, Shaneann R Fross, Xiyu Liu, Lu Diao, Karena Liang, Jinxuan Wu, Qingfei Wang, Jacqueline A Lopez and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Samantha M GolombDepartment of Pathology, UT Southwestern Medical Center, Dallas, TX 75390, USA; Harold C. Simmons Comprehensive Cancer Center, Dallas, TX 75390, USA; Department of Biological Sciences, College of Science, University of Notre Dame, Notre Dame, IN 46556, USA; Mike and Josie Harper Cancer Research Institute, University of Notre Dame, 1234 N. Notre Dame Avenue, South Bend, IN 46617, USA.
Ian H GuldnerDepartment of Biological Sciences, College of Science, University of Notre Dame, Notre Dame, IN 46556, USA; Mike and Josie Harper Cancer Research Institute, University of Notre Dame, 1234 N. Notre Dame Avenue, South Bend, IN 46617, USA.
Emilija AleksandrovicDepartment of Pathology, UT Southwestern Medical Center, Dallas, TX 75390, USA; Harold C. Simmons Comprehensive Cancer Center, Dallas, TX 75390, USA; Department of Biological Sciences, College of Science, University of Notre Dame, Notre Dame, IN 46556, USA; Mike and Josie Harper Cancer Research Institute, University of Notre Dame, 1234 N. Notre Dame Avenue, South Bend, IN 46617, USA.
Shaneann R FrossDepartment of Pathology, UT Southwestern Medical Center, Dallas, TX 75390, USA; Harold C. Simmons Comprehensive Cancer Center, Dallas, TX 75390, USA; Department of Biological Sciences, College of Science, University of Notre Dame, Notre Dame, IN 46556, USA; Mike and Josie Harper Cancer Research Institute, University of Notre Dame, 1234 N. Notre Dame Avenue, South Bend, IN 46617, USA.
Xiyu LiuDepartment of Pathology, UT Southwestern Medical Center, Dallas, TX 75390, USA; Harold C. Simmons Comprehensive Cancer Center, Dallas, TX 75390, USA; Department of Biological Sciences, College of Science, University of Notre Dame, Notre Dame, IN 46556, USA; Mike and Josie Harper Cancer Research Institute, University of Notre Dame, 1234 N. Notre Dame Avenue, South Bend, IN 46617, USA.
Lu DiaoDepartment of Pathology, UT Southwestern Medical Center, Dallas, TX 75390, USA; Harold C. Simmons Comprehensive Cancer Center, Dallas, TX 75390, USA.
Karena LiangDepartment of Pathology, UT Southwestern Medical Center, Dallas, TX 75390, USA.
Jinxuan WuDepartment of Pathology, UT Southwestern Medical Center, Dallas, TX 75390, USA.
Qingfei WangDepartment of Biological Sciences, College of Science, University of Notre Dame, Notre Dame, IN 46556, USA; Mike and Josie Harper Cancer Research Institute, University of Notre Dame, 1234 N. Notre Dame Avenue, South Bend, IN 46617, USA.
Jacqueline A LopezDepartment of Biological Sciences, College of Science, University of Notre Dame, Notre Dame, IN 46556, USA.
Siyuan ZhangDepartment of Pathology, UT Southwestern Medical Center, Dallas, TX 75390, USA; Harold C. Simmons Comprehensive Cancer Center, Dallas, TX 75390, USA; Department of Biological Sciences, College of Science, University of Notre Dame, Notre Dame, IN 46556, USA; Mike and Josie Harper Cancer Research Institute, University of Notre Dame, 1234 N. Notre Dame Avenue, South Bend, IN 46617, USA. Electronic address: siyuan.zhang@utsouthwestern.edu.

Funding

Neural Niche in Promoting Brain Metastatic Tumor ProgressionR01CA255064 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI ZHANG, SIYUAN · 2021 to 2025
$2.3M
Mechanisms of neuroinflammation in brain metastasis progressionR01CA222405 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI ZHANG, SIYUAN · 2018 to 2022
$2.2M
(PQD-3) Spatiotemporal Molecular Interrogation of Early Metastatic Evolution In SituR01CA194697 · NCI · UNIVERSITY OF NOTRE DAME · PI ZHANG, SIYUAN · 2015 to 2018
$1.4M
Prevention of Brain Metastasis Relapse through Modulation of Age-related Gut Microbiota-Immune Cross talkR21CA263798 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI ZHANG, SIYUAN · 2021 to 2022
$400k
Dissecting Gut Microbiota Modulation on Brain Metastasis ProgressionF31CA261046 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI GOLOMB, SAMANTHA M · 2021 to 2024
$147k
NCI NIH HHS F31 CA261046NCI NIH HHS R01 CA194697NCI NIH HHS R01 CA222405NCI NIH HHS R01 CA255064NCI NIH HHS R21 CA263798
6 · The paper itself

Abstract

Interactions between metastatic cancer cells and the brain microenvironment regulate brain metastasis (BrMet) progression. Central nervous system (CNS)-native and peripheral immune cells influence the BrMet immune landscape, but the dynamics and factors modulating this microenvironment remain unclear. As the gut microbiome impacts CNS and peripheral immune activity, we investigated its role in regulating immune response dynamics throughout BrMet stages. Antibiotic-induced (ABX) gut dysbiosis significantly increased BrMet burden versus controls but was equalized with fecal matter transplantation, highlighting microbiome diversity as a regulator of BrMet. Single-cell sequencing revealed a highly dynamic immune landscape during BrMet progression in both conditions. However, the timing of the monocyte inflammatory response was altered. Microglia displayed an elevated activation signature in late-stage metastasis in ABX-treated mice. T cell and microglia perturbation revealed involvement of these cell types in modulating BrMet under gut dysbiosis. These data indicate profound effects on immune response dynamics imposed by gut dysbiosis across BrMet progression.

Indexed as

Brain NeoplasmsDysbiosisGastrointestinal MicrobiomeTranscriptomeAnimalsDisease ProgressionFemaleHumansMiceMice, Inbred C57BLMicrogliaT-Lymphocytesbrain metastasiscancerCP: CancerCP: Microbiologygut microbiomesingle cell RNA sequencing

Identifiers

PMID40023843
PMCPMC12028778

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.