ArticleAMB Express2025
A method for creating in-frame insertions of fluorescent proteins in non-model gram-negative bacteria.
Article in AMB Express, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- DNA damage-associated vesicle production inJournal of bacteriology · 2026Article
- Correction: A method for creating in-frame insertions of fluorescent proteins in non-model gram-negative bacteria.AMB Express · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
Abstract
The use of fluorescent proteins to study protein expression and localisation has become common practice in the life sciences. While methods to create gene fusions and replacements with fluorescent proteins in model organisms have rapidly developed, there exist far fewer well-established protocols applicable to non-model bacteria. Here, we present a comprehensive account of an allelic-exchange-based mutagenesis strategy using the I-SceI endonuclease in a clinical strain of S. maltophilia. We demonstrate the use of this strategy for the creation of in-frame insertions of fluorescent proteins and entire gene replacements for the purposes of studying protein localisation and expression. This protocol requires minimal setup, and once optimised, can produce mutants in a matter of weeks. We expect this strategy to be of use for laboratories working with poorly-characterised strains and/or bacteria for which information is scarce.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.