Biosynthesis of the C-Glycosylated Depside Arenicolin B.

Colin W Johnson, Lorraine Nuniz, Bruno Perlatti, Gerald F Bills, Yi Tang

Abstract read

In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Heterologous expression of anChemical science · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Colin W JohnsonDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, 90095, United States.

Lorraine NunizDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, 90095, United States.

Bruno PerlattiDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, 90095, United States.

Gerald F BillsTexas Therapeutics Institute, The Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, Houston, Texas, 77054, United States.

Yi TangDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, 90095, United States.ORCID https://orcid.org/0000-0003-1597-0141

Funding

MIRA: Enzymology and Self-Resistance of Natural Product BiosynthesisR35GM118056 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Yi Tang · 2016 to 2026

$6.1M

NIGMS NIH HHS R35 GM118056NIGMS NIH HHS R35GM118056

6 · The paper itself

Abstract

We uncovered the biosynthetic pathway of the C-glycosylated depside arenicolin B from Phialomyces arenicola through genome mining, heterologous reconstitution and biochemical characterization. The biosynthetic gene cluster (BGC) of arenicolin B is comprised of a HRPKS, a NRPKS, and a C-glycosyltransferase. Through biochemical dissection and evaluation, we demonstrate the NRPKS thioesterase (TE) domain catalyses depside formation.

Indexed as

AscomycotaBiosynthetic PathwaysGlycosylationMultigene FamilyC-glycosyltransferasedepside natural productglycosylated natural productnatural product biosynthesispolyketide synthase

Identifiers

PMID40020202

DOI10.1002/cbic.202500003

PMCPMC12143958

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.