ArticleVeterinary medicine and science2025
Impact of Selected Natural Bioactive Substances on Immune Response and Tight Junction Proteins in Broiler Chickens.
Article in Veterinary medicine and science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Distinct Host and Bacterial Profiles in Murine Single Versus Co-Infection with Foal-IsolatedMicroorganisms · 2026Article
- Article
- Spleen Metabolome Reveals Immune-Mediated Responses Modulated by Onion Peel Extract inMicroorganisms · 2026Article
- Architecture, permeability, disruption dynamics and remodeling of tight junction proteins: Implications for performance, health, and welfare of the broiler chicken.Poultry science · 2026Review
- Synergistic effects of dietary RISCO-NUTRIFOUR probiotic on broiler performance, upregulation of intestinal immunoglobulin A and mucin-2 genes, enhancement of occludin expression, and downregulation of HSP70 under heat stress.Frontiers in veterinary science · 2026Article
- Antibacterial effects of epigallocatechin gallate against calf diarrhea-causingFrontiers in veterinary science · 2026Article
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Authors and funding
9 authors.
Funding
Abstract
This study was conducted to examine the effect of dietary natural compounds, such as β-glucan, carotenoids, oligosaccharides and anthocyanins, on immune response and tight junction proteins in broiler chickens. A total of 900 one-day-old chickens were allocated to five treatments in three floor pens (replicates) of 60 broilers per pen. Chickens were fed five diets: a control (basal) diet, a diet supplemented with β-glucan at 0.05%, or diets supplemented with carotenoids, oligosaccharides or anthocyanins at 0.5% of each compound. Male broilers were randomly selected for sample collections. On Day 25, plasma samples were collected from the brachial vein. On Day 26, six broilers were intraperitoneally injected with 2 mg of lipopolysaccharide per kg of body weight. Twelve hours later (Day 27), blood and ileum samples were collected to determine immune parameters and tight junction proteins using ELISA assays. The results showed that anthocyanin supplementation reduced the level of interleukin-1β compared to the lipopolysaccharide-injected control group (p = 0.047), which suggests that anthocyanin could partly alleviate the inflammation. Carotenoids reached a lower level of interleukin-6 compared to the β-glucan treatment (p = 0.0466). β-Glucan (p = 0.0382) and oligosaccharides (p = 0.0449) increased the level of plasma immunoglobulin G compared to the challenged control group, which may indicate an enhanced humoral immunity. Furthermore, β-glucan (except for occludin 2), carotenoids, oligosaccharides and anthocyanins increased (p < 0.05) the levels of ileal zonula occludens-1, occludin 1 and occludin 2 compared to the lipopolysaccharide-challenged control chickens. This may suggest that all the bioactive substances improved the gut barrier function. The plasma levels of tight junction proteins show higher concentrations in lipopolysaccharide-challenged groups compared to the non-challenged groups (p < 0.05). This may refer to the tight junction disruption and appearance in circulation as a reflection of lipopolysaccharide exposure.
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