Evidence map›Paper›PMID 40019273›Full record

ArticleApplied and environmental microbiology2025

CARPDM: cost-effective antibiotic resistome profiling of metagenomic samples using targeted enrichment.

Dirk Hackenberger, Hamna Imtiaz, Amogelang R Raphenya, Brian P Alcock, Hendrik N Poinar, Gerard D Wright, Andrew G McArthur

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Population structure, resistome, and virulome ofFrontiers in cellular and infection microbiology · 2025
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Dirk HackenbergerM.G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
Hamna ImtiazM.G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
Amogelang R RaphenyaM.G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
Brian P AlcockM.G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
Hendrik N PoinarM.G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
Gerard D WrightM.G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.ORCID 0000-0002-9129-7131
Andrew G McArthurM.G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.ORCID 0000-0002-1142-3063

Funding

Canadian Government | Canadian Institutes of Health Research (CIHR) PJT-156214,PJT-183745
6 · The paper itself

Abstract

Better interrogation of antimicrobial resistance requires new approaches to detect the associated genes in metagenomic samples. Targeted enrichment is an ideal method for their sequencing and characterization. However, no open-source, up-to-date hybridization probe set targeting antimicrobial resistance genes exists. Here, we describe the Comprehensive Antibiotic Resistance Probe Design Machine (CARPDM), a probe design software package made to run alongside all future Comprehensive Antibiotic Resistance Database releases. To test its efficacy, we have created and tested two separate probe sets: allCARD, which enriches all genes encoded in the Comprehensive Antibiotic Resistance Database's protein homolog models (

Indexed as

Anti-Bacterial AgentsBacteriaDrug Resistance, BacterialMetagenomeMetagenomicsSoftwareCost-Benefit AnalysisAnti-Bacterial Agentsantimicrobial resistancemetagenomicssequencingtarget enrichment

Identifiers

PMID40019273
PMCPMC11921354

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.