Evidence map›Paper›PMID 40018956›Full record

ArticleJournal of water and health2025

Multi-tiered strategy for large-scale wastewater detection of SARS-CoV-2 in low-case settings provides confidence for public health actions.

Aaron Jex, Nijoy John, David McCarthy, Steven Myers, Natacha Begue, Christelle Schang, Shane P Usher, Louise Baker, Christine Kaucner, Paul Monis and 17 more

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of water and health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

27 authors.

Aaron JexInfection and Global Health, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia; Faculty of Science, The University of Melbourne, Parkville, Victoria 3010, Australia.
Nijoy JohnInfection and Global Health, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia; Faculty of Science, The University of Melbourne, Parkville, Victoria 3010, Australia E-mail: john.n@wehi.edu.au.ORCID https://orcid.org/0000-0002-6376-2630
David McCarthyEnvironmental and Public Health Microbiology Laboratory (EPHM Lab), Department of Civil Engineering, Monash University, Clayton, Victoria 3800, Australia; School of Civil and Environmental Engineering, Queensland University of Technology, Brisbane 4000, Australia.
Steven MyersDepartment of Microbiology, Australian Laboratory Services, Scoresby, Victoria 3179, Australia.
Natacha BegueDepartment of Microbiology, Australian Laboratory Services, Scoresby, Victoria 3179, Australia.
Christelle SchangEnvironmental and Public Health Microbiology Laboratory (EPHM Lab), Department of Civil Engineering, Monash University, Clayton, Victoria 3800, Australia.
Shane P UsherDepartment of Chemical Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.
Louise BakerInfection and Global Health, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia.
Christine KaucnerDepartment of Microbiology, Australian Laboratory Services, Scoresby, Victoria 3179, Australia.
Paul MonisEnvironment and Wastewater, Australian Water Quality Centre, SA Water, Adelaide, South Australia 5000, Australia.
Leon M HartmanInfection and Global Health, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia.
Brianna FlynnInfection and Global Health, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia.
James E MerrettInfection and Global Health, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia.
David ListerDepartment of Health, State Government of Victoria, Melbourne, Victoria 3000, Australia.
Marco HeroldInfection and Global Health, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia; School of Cancer Medicine, Olivia Newton-John Cancer Research Institute, La Trobe University, Heidelberg, Victoria 3084, Australia.
Andrew KuehInfection and Global Health, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia; School of Cancer Medicine, Olivia Newton-John Cancer Research Institute, La Trobe University, Heidelberg, Victoria 3084, Australia.
Ngai Ning ChengEnvironment and Wastewater, Australian Water Quality Centre, SA Water, Adelaide, South Australia 5000, Australia; Department of Health, State Government of Victoria, Melbourne, Victoria 3000, Australia.
Jackson NolanDepartment of Health, State Government of Victoria, Melbourne, Victoria 3000, Australia.
Leon CalyVictorian Infectious Diseases Reference Laboratory, Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.
Julian DruceVictorian Infectious Diseases Reference Laboratory, Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.
Bruce ThorleyVictorian Infectious Diseases Reference Laboratory, Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.
Peter J ScalesDepartment of Chemical Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.
Jonathan SchmidtSouth East Water Corporation, Frankston, Victoria 3199, Australia.
Suzie SarkisDepartment of Health, State Government of Victoria, Melbourne, Victoria 3000, Australia.
Nicholas D CrosbieRecycled Water and Integrated Water Management, Melbourne Water Corporation, Docklands, Victoria 3001, Australia.
Rachael PoonDepartment of Health, State Government of Victoria, Melbourne, Victoria 3000, Australia.
Monica NolanDepartment of Health, State Government of Victoria, Melbourne, Victoria 3000, Australia.

Funding

Department of Health, State Government of Victoria 2064National Health and Medical Research Council APP1194330Water Research Australia 2064
6 · The paper itself

Abstract

Wastewater surveillance has played a pivotal role in monitoring SARS-CoV-2 transmission worldwide. However, developing and implementing the methods underpinning these programmes in regions with prolonged periods of low community transmission has proven challenging. In Victoria, Australia, wastewater surveillance provided early warning of unknown community infections and informed timely public health decisions to limit their spread when case numbers were low. To achieve this, we developed a methodological approach sensitive to extremely low viral loads and could readily identify false positives within short turnaround times. Here, we describe the successful development, implementation, and evaluation of analytic methods using Reverse Transcriptase Quantitative Polymerase Chain Reaction (RT-qPCR) and amplicon sequencing in tandem with CRISPR DETECTR in an ongoing, large-scale surveillance programme to detect SARS-CoV-2 in wastewater in Victoria, Australia. Our study covers ten months, from July 2020 to April 2021, and includes all state-wide health districts and prolonged periods with no known, active community cases among the ∼6.7 million population.

Indexed as

COVID-19SARS-CoV-2WastewaterHumansPublic HealthVictoriaWastewateramplicon sequencingcommunity transmissionCRISPR DETECTRSARS-CoV-2wastewater surveillance

Identifiers

PMID40018956

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.