Evidence map›Paper›PMID 40017741›Full record

ArticleJACS Au2025

Biochemistry and Chemoinformatics Guided Classification of Hirsutane Sesquiterpenes Isolated from Mushroom.

Yaping Liu, Jumpei Nishishita, Chengwei Liu, Hideaki Oikawa, Atsushi Minami

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PubMed Full text at PMC Publisher
In one paragraph

Article in JACS Au, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yaping LiuDepartment of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan.
Jumpei NishishitaDepartment of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan.
Chengwei LiuKey Laboratory for Enzyme and Enzyme-like Material Engineering of Heilongjiang, College of Life Science, Northeast Forestry University, Harbin 150040, China.ORCID https://orcid.org/0000-0003-2746-2080
Hideaki OikawaDepartment of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan.ORCID https://orcid.org/0000-0002-6947-3397
Atsushi MinamiDepartment of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan.ORCID https://orcid.org/0000-0002-4183-0268

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hirsutanes are mushroom-derived sesquiterpenes with a characteristic 5-5-5 tricyclic ring skeleton. To date, more than 70 derivatives have been isolated from nature. In this study, we applied heterologous expression, in vitro enzymatic reactions, and biotransformation to characterize the function of nine enzymes involved in the synthesis of anhydroarthrosporone and six novel hirsutanes. The elucidated biosynthesis involves oxidative modifications of the A-ring followed by structural diversification of the B- and C-rings. Most importantly, biosynthetic pathways provide crucial insights into the classification and organization of isolated hirsutanes. We successfully classified 69 natural hirsutanes into three groups based on their A-ring modification patterns. Our classification covered 92% of the natural hirsutanes. A comprehensive understanding of their biosynthesis will provide opportunities to isolate structurally diverse hirsutanes using genetic engineering techniques.

Identifiers

PMID40017741
PMCPMC11862936

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.