Evidence map›Paper›PMID 40016457›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Quantifying Rhinovirus Genomic and Negative-Strand RNA by RT-PCR.

Shahina Wiehler, Michelle E Love, David Proud

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Shahina WiehlerDepartment of Physiology and Pharmacology, and Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Michelle E LoveDepartment of Physiology and Pharmacology, and Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
David ProudDepartment of Physiology and Pharmacology, and Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. dproud@ucalgary.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Molecular methods permit measurements of viral genomic RNA as well as of the negative-strand template generated during rhinovirus replication. As such, assays of negative-strand RNA can provide a means for relative quantification of levels of double-stranded RNA that is generated during replication and that interacts with pattern recognition receptors to activate the host immune response. Here we provide RT-PCR protocols for quantitative assays for rhinovirus genomic and negative-strand RNAs.

Indexed as

Genome, ViralReverse Transcriptase Polymerase Chain ReactionRhinovirusRNA, ViralHumansRNA, Double-StrandedVirus ReplicationRNA, Double-StrandedRNA, ViralAirway epitheliumHuman rhinovirusNegative-strand RNAReal-time PCR genomic RNA

Identifiers

PMID40016457

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.