Evidence map›Paper›PMID 40016456›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Quantification of Infectious Rhinovirus A and B Serotypes by Plaque Assay.

Mohsen Tabasi, Haleh Ganjian, Umadevi Sajjan

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Mohsen TabasiCentre for Inflammation and Lung Research, Lewis Katz Medical School, Temple University, Philadelphia, PA, USA.
Haleh GanjianCentre for Inflammation and Lung Research, Lewis Katz Medical School, Temple University, Philadelphia, PA, USA.
Umadevi SajjanCentre for Inflammation and Lung Research, Lewis Katz Medical School, Temple University, Philadelphia, PA, USA. uma.sajjan@temple.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plaque assay is a quantitative assay that determines the number of infective virions in the viral stock or the infected cells. Plaques are essentially virus-infected cells that produce progeny virus and infect adjacent cells. The cells producing progeny virus die and detach from the dish, leaving an empty space in the confluent monolayer of cells. Unlike other viruses, rhinovirus does not form characteristic round plaques but shows small clear areas of different shapes surrounded by dying cells. Rhinoviruses form plaques only in highly susceptible H1HeLa cells but not in their primary target, airway epithelial cells. The plaque assay to determine the number of infective virions works only for rhinovirus species A and B, but not C, because the latter does not infect H1HeLa cells. Here we describe a method to quantify the infective virions by plaque assay for rhinovirus speciesA and B.

Indexed as

RhinovirusViral Plaque AssayCell LineHumansPicornaviridae InfectionsSerogroupVirionAirway epithelial cellsCell deathCrystal violetH1HeLa cellsTCID50Virus infectivity assay

Identifiers

PMID40016456

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.