Evidence map›Paper›PMID 40014012›Full record

ArticleMicrobiology spectrum2025

Novel application of ribonucleoprotein-mediated CRISPR-Cas9 gene editing in plant pathogenic oomycete species.

Erika N Dort, Nicolas Feau, Richard C Hamelin

Abstract read
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Erika N DortDepartment of Forest & Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, British Columbia, Canada.ORCID 0000-0002-7814-1060
Nicolas FeauPacific Forestry Centre, Canadian Forest Service, Natural Resources Canada, Victoria, British Columbia, Canada.ORCID 0000-0001-5925-9867
Richard C HamelinDepartment of Forest & Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, British Columbia, Canada.

Funding

Canadian Government | Natural Sciences and Engineering Research Council of Canada (NSERC) CGS-DGenome Canada (GC) 10106
6 · The paper itself

Abstract

CRISPR-Cas9 gene editing has become an important tool for the study of plant pathogens, allowing researchers to functionally characterize specific genes involved in phytopathogenicity, virulence, and fungicide resistance. Protocols for CRISPR-Cas9 gene editing have already been developed for Phytophthoras, an important group of oomycete plant pathogens; however, these efforts have exclusively focused on agricultural pathosystems, with research lacking for forest pathosystems. We sought to develop CRISPR-Cas9 gene editing in two forest pathogenic Phytophthoras,

Indexed as

CRISPR-Cas SystemsGene EditingPhytophthoraRibonucleoproteinsFungicides, IndustrialHydrocarbons, FluorinatedOomycetesPlant DiseasesPyrazolesVirulenceFungicides, IndustrialHydrocarbons, FluorinatedoxathiapiprolinPyrazolesRibonucleoproteinsantifungal resistanceforest pathologygene disruptionPhytophthoraplasmid co-transformation

Identifiers

PMID40014012
PMCPMC11960053

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.