Evidence map›Paper›PMID 40013512›Full record

ReviewPlant biotechnology journal2025

Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.

Nikita Gondalia, Luis Felipe Quiroz, Linyi Lai, Avinash Kumar Singh, Moman Khan, Galina Brychkova, Peter C McKeown, Manash Chatterjee, Charles Spillane

Abstract readReview
In one paragraph

Review in Plant biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
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  5. Genome-Wide Identification of theInternational journal of molecular sciences · 2026
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  7. Article
  8. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Nikita Gondalia *Agriculture, Food Systems and Bioeconomy Research Centre, Ryan Institute, University of Galway, Galway, Ireland.ORCID https://orcid.org/0000-0002-5518-6274
Luis Felipe Quiroz *Agriculture, Food Systems and Bioeconomy Research Centre, Ryan Institute, University of Galway, Galway, Ireland.ORCID https://orcid.org/0000-0002-7907-7320
Linyi LaiAgriculture, Food Systems and Bioeconomy Research Centre, Ryan Institute, University of Galway, Galway, Ireland.ORCID https://orcid.org/0000-0001-8815-6752
Avinash Kumar SinghAgriculture, Food Systems and Bioeconomy Research Centre, Ryan Institute, University of Galway, Galway, Ireland.ORCID https://orcid.org/0000-0001-6572-6732
Moman KhanAgriculture, Food Systems and Bioeconomy Research Centre, Ryan Institute, University of Galway, Galway, Ireland.ORCID https://orcid.org/0000-0003-3432-6933
Galina BrychkovaAgriculture, Food Systems and Bioeconomy Research Centre, Ryan Institute, University of Galway, Galway, Ireland.ORCID https://orcid.org/0000-0003-2452-7635
Peter C McKeownAgriculture, Food Systems and Bioeconomy Research Centre, Ryan Institute, University of Galway, Galway, Ireland.ORCID https://orcid.org/0000-0002-7255-6062
Manash ChatterjeeAgriculture, Food Systems and Bioeconomy Research Centre, Ryan Institute, University of Galway, Galway, Ireland.ORCID https://orcid.org/0009-0002-7843-2826
Charles SpillaneAgriculture, Food Systems and Bioeconomy Research Centre, Ryan Institute, University of Galway, Galway, Ireland.ORCID https://orcid.org/0000-0003-3318-323X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genome-edited plants, endowed with climate-smart traits, have been promoted as tools for strengthening resilience against climate change. Successful plant gene editing (GE) requires precise regulation of the GE machinery, a process controlled by the promoters, which drives its transcription through interactions with transcription factors (TFs) and RNA polymerase. While constitutive promoters are extensively used in GE constructs, their limitations highlight the need for alternative approaches. This review emphasizes the promise of tissue/organ specific as well as inducible promoters, which enable targeted GE in a spatiotemporal manner with no effects on other tissues. Advances in synthetic biology have paved the way for the creation of synthetic promoters, offering refined control over gene expression and augmenting the potential of plant GE. The integration of these novel promoters with synthetic systems presents significant opportunities for precise and conditional genome editing. Moreover, the advent of bioinformatic tools and artificial intelligence is revolutionizing the characterization of regulatory elements, enhancing our understanding of their roles in plants. Thus, this review provides novel insights into the strategic use of promoters and promoter editing to enhance the precision, efficiency and specificity of plant GE, setting the stage for innovative crop improvement strategies.

Indexed as

Gene EditingPlantsPromoter Regions, GeneticCRISPR-Cas SystemsGene Expression Regulation, PlantGenome, PlantPlants, Genetically ModifiedCRISPR/Casplant gene editingpromoter editingPromoterssynthetic biologysynthetic promoters

Identifiers

PMID40013512
PMCPMC12018835

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.