ArticleCureus2025
The Dysregulation of microRNA-30b Directly Regulates Cystathionine Gamma-Lyase and Exhibits Poor Invasion Activity in Preeclampsia.
Article in Cureus, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Targeting the microbiota-miRNA-protease axis: A new therapeutic avenue in melanoma.The FEBS journal · 2026Review
- Hydrogen sulfide in placental development and pregnancy disorders: mechanisms, therapeutic potential, and translational challenges.Reproductive biology and endocrinology : RB&E · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background and objective Preeclampsia (PE) is a severe pregnancy complication characterized by high blood pressure and proteinuria after 23 weeks of gestation. Insufficient trophoblast invasion has been linked to its development. Hydrogen sulfide (H₂S), with its genes cystathionine gamma-lyase (CTH) playing the role of an important vasodilator, may be associated with various diseases including PE. This study investigated the role of microRNA-30b (miR-30b) in trophoblast invasion using HTR-8/SVneo cells, blood, and placental samples Methods The study involved samples from 40 preeclamptic subjects and 40 healthy controls. Gene expression was analyzed via quantitive real-time PCR and enzyme-linked immunosorbent assay (ELISA). The effects of H₂S were simulated using a sodium hydrogen sulfide (NaHS) inhibitor (AOAA), and miR-30b inhibitors were used to assess changes in invasion capacity. Results NaHS treatment enhanced trophoblast invasion, and hypoxia/reoxygenation (H/R) + NaHS-treated cells showed increased invasion compared to H/R-treated cells alone. miR-30b inhibition led to higher expression of CTH, matrix metalloproteinase-9 (MMP-9), and reduced tissue inhibitor of metalloproteinases 1 (TIMP-1) and TIMP-2 expression, improving cell invasion. Patient samples showed lower CTH, MMP-2, and MMP-9 levels in PE, with elevated TIMP-1 and TIMP-2. Protein expression also revealed reduced CTH and MMP-9 in preeclamptic patients. Conclusions Based on our findings, miR-30b influences trophoblast invasion by modulating CTH expression and the MMP/TIMP balance. Enhanced H₂S production improves invasion, suggesting that miR-30b and related pathways can be potential therapeutic targets for PE management.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.