ArticleCureus2025
Assessment of Umbilical Cord Mesenchymal Stem Cell Cultivation Using Fetal Bovine Serum or Platelet Lysate.
Article in Cureus, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Enhance Chondrocyte Function by Reducing Oxidative Stress in Chondrocytes.International journal of molecular sciences · 2025Article
- Quality by design strategy of human mesenchymal stem/stromal cell drug products for the treatment of knee osteoarthritis.World journal of stem cells · 2025Review
- Immunophenotypic characterization and clinical relevance of passage number in umbilical cord mesenchymal stromal cells: A review.Cell transplantationReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWharton's jelly (WJ) and umbilical cord blood (UCB) are considered marvelous sources of mesenchymal stem cells (MSCs) due to their availability, simply isolated without pain and ethical issues. Additionally, UC-MSCs are more primitive than MSCs isolated from adult sources, thus opening new possibilities for cell therapies.
objectiveIn this study, we aimed to develop a simple, economical, and efficient xenogenic-free protocol for the isolation and expansion of UC-MSCs to be compliant with good manufacturing practice (GMP) guidelines. METHODS/
designIn this work, we used the explant-partial enzymatic digestion technique for WJ-MSCs and Ficoll-Paque density centrifugation for CB-MSC isolation. Human platelet lysate (HPL) was used as a replacement for fetal bovine serum (FBS), which is used in most protocols.
resultsWe observed that the explants-partial enzymatic digestion technique is an effective protocol for MSC isolation from WJ, and HPL is a safe, powerful, and cost-effective substitute for FBS in the MSC propagation. Among all tested conditions, 10% HPL demonstrated the best growth rate, highest viability count, and highest expression level of a cluster of differentiation markers (CD).
conclusionWe concluded that WJ-MSCs are superior to UCB-MSCs in the matter of rapidity and homogeneity, and both of them expressed MSC-positive markers CD44 and CD73, suggesting that WJ is a source with greater potential for MSC isolation for clinical application.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.