Evidence map›Paper›PMID 40013121›Full record

ArticleAnimal reproduction2025

L-Carnitine enhances porcine sperm quality, longevity, and zona pellucida binding in cooled semen.

Monique de Albuquerque Lagares, Nathalia Abreu Amaral, Joyce Junia Braga, Natalia de Castro Alves, Marina Morra Freitas, Rafael Romero Nicolino, Raphael Rocha Wenceslau, Fernanda da Rocha Anselmo, Marina Maria do Carmo Silva Oliveira, Eduardo Damasceno Costa and 2 more

Abstract read
In one paragraph

Article in Animal reproduction, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Monique de Albuquerque LagaresEscola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.ORCID 0000-0003-1795-6984
Nathalia Abreu AmaralInstituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.ORCID 0000-0002-2703-1654
Joyce Junia BragaInstituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.ORCID 0000-0003-2966-3644
Natalia de Castro AlvesEscola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.ORCID 0000-0002-6460-5856
Marina Morra FreitasEscola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.ORCID 0000-0002-9742-3426
Rafael Romero NicolinoEscola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.ORCID 0000-0001-5945-9949
Raphael Rocha WenceslauEscola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.ORCID 0000-0002-0034-1484
Fernanda da Rocha AnselmoDepartamento de Medicina Veterinária, Pontifícia Universidade Católica, Betim, MG, Brasil.ORCID 0009-0008-1610-8450
Marina Maria do Carmo Silva OliveiraDepartamento de Medicina Veterinária, Pontifícia Universidade Católica, Betim, MG, Brasil.ORCID 0009-0007-5276-8379
Eduardo Damasceno CostaInstituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.ORCID 0000-0003-4769-5308
Fernanda Radicchi Campos Lobato de AlmeidaInstituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.ORCID 0000-0003-4219-1290
Rubens StahlbergDepartamento de Medicina Veterinária, Pontifícia Universidade Católica, Betim, MG, Brasil.ORCID 0009-0003-8680-2783

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine breeding industries typically ensure the viability of boar artificial insemination doses during a 5-day liquid storage period at 17 °C. This study aimed to investigate whether the addition of L-carnitine (LC) to boar semen doses on different days of cooled storage could extend their usability. In experiment 1, LC was added to porcine semen doses on the fifth day (d5) of cooled storage performing five treatments control (no LC), 0.5, 1-, 5- and 10-mM LC. On d6 and d8 of storage, semen samples were evaluated for sperm motility and kinematic parameters, membrane functionality, and hydrogen peroxide and nitrite concentrations. In experiment 2, the number of sperm bound to the zona pellucida (ZP) was determined, as a way to investigate sperm penetration capability from boar insemination doses, with co-incubation with porcine oocytes. LC concentration that produced the most favorable outcomes in Experiment 1 was chosen to experiments 2 and 3, performing two treatments in the absence and with the LC. In Experiment 3, LC was added to cooled porcine semen doses after one day of storage (d1), and the same evaluations of experiment 1 were conducted on days 5, 7, 9, and 12, including sperm membrane integrity. The addition of 10 mM LC on d5 and d1 of storage improved sperm motility, which was extended up to 8 and 12 days of cooled storage, respectively. LC addition on d5 of storage increased sperm membrane functionality, while when added to semen on d1 of storage, it decreased NO

Indexed as

antioxidantartificial inseminationboarspermatozoaswine

Identifiers

PMID40013121
PMCPMC11864729

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.