ArticleBMC microbiology2025
Characterization and genome analysis of novel Klebsiella pneumoniae phage vbKpUKJ_2 isolated from hospital sewage water.
Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Bacteriophage cocktails and bacterial coevolution: a systematic review of resistance prevention strategies against multidrug-resistant pathogens in sewage water.Iranian journal of microbiology · 2026Article
- Phage-antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance.Journal of biomedical science · 2026Article
- Genomic and functional characterization of a novel halophilic bacteriophage targeting carbapenem-resistant Klebsiella pneumoniae.PloS one · 2026Article
- Phage and endolysin-based inhibition ofFrontiers in cellular and infection microbiology · 2026Review
- A novel bacteriophage capable of efficiently lysing multi-drug-resistantFrontiers in microbiology · 2026Article
- A lytic bacteriophage vB_KpnP-6K2 inhibits ST11-KL64Frontiers in cellular and infection microbiology · 2026Article
- Isolation and characterization of an antibiofilm bacteriophage against Klebsiella michiganensis and evaluation of its therapeutic potential in an insect larval gut colonization model.BMC microbiology · 2025Article
- Isolation, characterization and therapeutic evaluation of phage HHU1 against K2Frontiers in cellular and infection microbiology · 2025Article
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Authors and funding
6 authors.
Funding
Abstract
introductionMultidrug-resistant (MDR) Klebsiella pneumoniae is a critical healthcare challenge due to its extensive resistance to antibiotics and role in causing severe infections. Bacteriophages offer a promising alternative for targeting MDR pathogens. This study characterizes a novel phage, vbKpUKJ_2, isolated from hospital sewage water, against clinical K. pneumoniae isolates.
methodsPhage vbKpUKJ_2 was isolated and purified using the double agar overlay method. Host range and sensitivity were tested against 40 clinical K. pneumoniae isolates using growth inhibition assays. Morphological characterization was performed using transmission electron microscopy (TEM). Genomic analysis was conducted to evaluate the absence of antibiotic resistance genes and determine phylogenetic relationships. Stability assays assessed the phage's thermal and pH tolerance.
resultsPhage vbKpUKJ_2 demonstrated broad range activity against clinical K. pneumoniae isolates. TEM revealed it belongs to the Drexlerviridae family. Genomic analysis confirmed the absence of antibiotic resistance genes and identified conserved functional regions shared with related phages. vbKpUKJ_2 exhibited broad pH stability (pH 4-10) and thermal stability between 30 °C and 60 °C. The one-step growth curve indicated rapid lytic activity, with a burst size of 323 phage particles per cell.
conclusionvbKpUKJ_2 shows promising therapeutic potential against MDR K. pneumoniae. Its stability, absence of resistance genes, and rapid lytic cycle highlight its suitability for inclusion in phage therapy protocols, particularly as part of combination therapies targeting MDR infections.
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