Evidence map›Paper›PMID 40009642›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

Leaky ribosomal scanning enables tunable translation of bicistronic ORFs in green algae.

Marco A Dueñas, Rory J Craig, Sean D Gallaher, Jeffrey L Moseley, Sabeeha S Merchant

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. The Elements of Life, Photosynthesis and Genomics.Journal of molecular biology · 2025
    Review
  7. A defined growth medium for trace metal limitation studies inbioRxiv : the preprint server for biology · 2025
    Article
  8. Gene regulation inCurrent research in parasitology & vector-borne diseases · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Marco A DueñasDepartment of Plant and Microbial Biology, University of California, Berkeley, CA 94720.ORCID 0000-0003-3822-6593
Rory J CraigCalifornia Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA 94720.ORCID 0000-0002-6262-0008
Sean D GallaherCalifornia Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA 94720.ORCID 0000-0002-9773-6051
Jeffrey L MoseleyCalifornia Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA 94720.ORCID 0000-0001-8603-8488
Sabeeha S MerchantDepartment of Plant and Microbial Biology, University of California, Berkeley, CA 94720.ORCID 0000-0002-2594-509X

Funding

Genetic Dissection of Cells and Organisms Training ProgramT32GM132022 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Nicole King, NOAH K WHITEMAN · 2019 to 2026
$5.2M
HHS | NIH | National Institute of General Medical Sciences (NIGMS) 1T32GM132022-01NIGMS NIH HHS T32 GM132022U.S. Department of Energy (DOE) DE-SC0023027
6 · The paper itself

Abstract

Advances in sequencing technology have unveiled examples of nucleus-encoded polycistrons, once considered rare. Exclusively polycistronic transcripts are prevalent in green algae, although the mechanism by which multiple polypeptides are translated from a single transcript is unknown. Here, we used bioinformatic and in vivo mutational analyses to evaluate competing mechanistic models for translation of bicistronic mRNAs in green algae. High-confidence manually curated datasets of bicistronic loci from two divergent green algae,

Indexed as

Chlamydomonas reinhardtiiChlorophytaOpen Reading FramesProtein BiosynthesisRibosomesCodon, InitiatorRNA, MessengerCodon, InitiatorRNA, MessengerdicistronicIso-Seqribosome profilingtransgene expressionuORFs

Identifiers

PMID40009642
PMCPMC11892635

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.